LAT1在前列腺癌中表达的意义

Clinical significance of LAT1 expression in prostate cancer tissues

目的 研究L型氨基酸转运子1（LAT1）在前列腺癌（PCa）中表达的意义.方法 用免疫组织化学方法检测LAT1在前列腺增生（BPH）组织和PCa组织中的表达,并分析LAT1的表达与PCa的病理分级、临床分期的关系.用基因芯片研究雄激素依赖性PCa细胞系C4与雄激素非依赖性PCa细胞系C4-2的基因表达差异,比较两个细胞系中LAT1mRNA的水平,并用免疫组织化学方法研究LAT1蛋白质在C4和C4-2中的表达.结果 LAT1在BPH和PCa组织中均有表达,BPH组织中LAT1的阳性表达率为77.0%,而在PCa组织中为95.5%,两者无统计学差异,但是在Pea组织中阳性染色的强度要明显高于BPH组织.LAT1的表达与前列腺癌的病理分级、临床分期无关.C4-2中LAT1 mRNA和蛋白质的表达水平明显高于C4.结论 LAT1的高表达可能与PCa的发病有关,也可能与PCa的雄激素非依赖化有关.

Objective To evaluate the clinical significance of LAT1 expression in prostate cancer tissues. Methods The expressions of LAT1 in benign prostate hyperplasia （BPH） tissues and prostate cancer tissue were detected by immunohistochemistry. The relationship between the expression of LATI and the clinical pathological grade of prostate cancer was explored. The genes expression of human androgen-dependent prostate cancer cells C4 and androgen-independent prostate cancer cells C4-2 were comparatively analyzed by gene chips containing LAT1. The expression of LAT1 protein in C4 and C4-2 cells was further confirmed by immunohistochemistry. Results There was no significant difference in the positive rate of LAT1 expression between BPH tissues and prostate cancer tissues. But the positive intensity of LAT1 in prostate cancer tissues was much stronger than that in BPH tissues. No relationship was found between the expressions of LAT1 and clinical pathological grades of PC. The mR_NA and protein expression of LAT1 were much higher in C4-2 cells than those in C4 cells. Conclusion High expression of LAT1 might be involved in prostate tumorigenesis and in the pathological transition of prostate cancer from androgen-dependent to androgen-independent.