菱角提取物诱导人肝癌SMMC-7721细胞凋亡

Trapa Extract Induced Apoptosis in Liver Cancer Cell Line SMMC-7721

[目的]探讨菱角提取物对人肝癌SMMC-7721细胞的增殖抑制及诱导凋亡作用。[方法]采用MTT法检测菱角提取物对SMMC-7721细胞的增殖抑制作用;丫啶橙染色、DNA琼脂糖凝胶电泳和流式细胞仪检测细胞凋亡。[结果]菱角提取物能抑制SMMC-7721细胞的增殖,且呈明显的剂量依赖性。荧光显微镜下细胞呈典型的凋亡形态学改变。[结论]菱角提取物可抑制SMMC-7721细胞的增殖并诱导其凋亡。

[Purpose] To investigate the anti-cell proliferation effect with Trapa extract in liver cancer cell line SMMC-7721.[Methods] The inhibition effect was detected by MTT method.Apoptosis was detected by AO staining,DNA agarose gel electrophoresis and FCM.[Results] The growth of SMMC-7721 cells was inhibited and cell apoptosis was induced by Trapa extract in a dose-dependent manner.Typical morphological changes of apoptosis were observed by fluorescence microscopy.[Conclusion] Trapa extract can inhibit proliferation and induce apoptosis in liver cancer cell line SMMC-7721.