微波消解-氢化物发生-原子荧光光谱法测定中药材中的痕量汞

Determination of trace mercury in Chinese herbal medicine by hydride generation-atomic fluorescence spectrometry with microwave digestion

目的:建立微波消解-氢化物发生-原子荧光光谱法测定中药材中痕量汞的方法。方法:用微波消解法对几种常用中药材样品进行前处理,用氢化物发生-原子荧光光谱法测定其中痕量汞。结果:微波消解过程中,消解试剂选择硝酸+双氧水,且按3∶1比例混合时消化效果最佳,汞在浓度为0μg/L^4μg/L范围内线性关系良好,相关系数r=0.9999,方法检出限为0.014μg/L,测定汞1.0μg/ml标准溶液相对标准偏差为1.99%,测定国家一级标准物质人发(GBW09101)相对标准偏差为3.37%,且测定结果与标准含量一致。结论:该方法测定中药材中的痕量汞简便快速,准确灵敏,易于推广。

Objective：To establish a method for determination of trace mercury in Chinese herbal medicine by hydride generation–atomic fluorescence spectrometry with microwave digestion.Methods： After pretreatment by microwave digestion system,trace mercury in several common Chinese herbal medicine were determined by hydride generation –atomic fluorescence spectrometry.Results： When using the nitric acid ＋ hydrogen peroxide reagent at the ratio of 3∶1 as acid digestion reagent in the digestion process,the samples will be completely digested.Wide linear relationship was achieved in the range of 0 μg/L～4 μg/L with relative coefficient of 0.9999 and the detection limit was 0.014 μg/L with RSD of 1.99% for mercury at 1.0 μg/L.Trace mercury in the standard reference substance human hair（GBW09101） was determined incording this way.The results were in good agreement with certified value and RSD was 3.37%.Conclusion： This method for the determination of trace mercury in Chinese herbal medicine is simple,rapid,sensitive,accurate and easy to use.