50株食源性金黄色葡萄球菌肠毒素特性及耐药分析

Enterotoxin property and drug resistance analysis of 50 strains of food-borne Staphylococcus aureus

目的:了解日常工作中分离出的食源性金黄色葡萄球菌产肠毒素特性及耐药性,为制定HACCP防止由金黄色葡萄球菌引起的食源性疾病及临床用药提供依据。方法:对分离得到的50株阳性菌株依据GB 4789.10-2010进行肠毒素A^E检验及分型;利用药敏分析仪对菌株进行药敏试验。结果:50株金黄色葡萄球菌中有20株肠毒素阳性,产肠毒素阳性率为40%,其中17株产了两种以上的毒素,有8株菌株产SEA;对氨苄西林(68%)、红霉素(56%)、克拉霉素(58%)、克林霉素(64%)、青霉素(88%)、四环素(38%)耐药率较高,并发现2株万古霉素耐药株;还发现葡萄球菌肠毒素阳性菌株对阿莫西林、苯唑西林+2%Nacl、链霉素、万古霉素耐药率较肠毒素阴性菌株高。结论:包装食品中有一定程度的金黄色葡萄球菌污染,尤其动物源性食品如冷冻食品、生肉制品等食品中较严重;食源性金黄色葡萄球菌产毒素能力较强,对肠毒素进一步分型更有实际意义;金黄色葡萄球菌对多种药物的耐药性提示安全应用治疗药物的重要性。

Objective：To understand the enterotoxin property and drug resistance of food-borne Staphylococcus aureus（S.aureus） isolated from food at daily work,and provide data for institution of HACCP,prevention of food-borne diseases caused by S.aureus and clinical drug therapy.Methods： With reference to GB 4789.10-2010,A～E staphylococcal enterotoxin-producing assay and antimicrobial susceptibility testing of 50 strains of isolated Positive strains were performed.Results： 20 strains of enterotoxin was detected in 50 S.aureus,and enterotoxin production was observed in 40%.17 strains have more than two kinds of drug,and 8 strains have SEA;S.aureus isolates were highly resistant to ampicillin（68%）,erythromycin（56%）,clarithromycin（58%）,clindamycin（64%）,penicillin（88%）,tetracycline（38%）,and two strains of resistant vancomycin was detected;it is founded that staphylococcal enterotoxin-producing have more resistance rate to amoxicillin,oxacillin ＋2% Nacl,streptomycin,vancomycin.Conclusion： Packaged foods are contaminated by S.aureus to some extent,especially in animal foods such as frozen foods,raw meat and so on.Food-borne S.aureus shows strong staphylococcal enterotoxin productivity,which has more practical meaning of Further classify.Multi-resistance shown by S.aureus isolated from food indicates that in the clinically therapeutic procedures well-targeted selection of antibiotics is advisable.