余杭区8起副溶血弧菌食物中毒的同源性研究

Study on homology of Vibrio parahaemolyticus isolated from the food poisoning in Yuhang

目的:应用PFGE分型技术,对食物中毒事件分离的副溶血弧菌株进行基因分型,分析各菌株间的同源性关系。方法:19株食物中毒来源的副溶血弧菌基因组DNA经限制性内切酶酶切,用脉冲场凝胶电泳方法获得电泳图谱,用B ioNum erics软件进行聚类分析。结果:19株副溶血弧菌的DNA指纹图谱,可大致分为9种PFGE图谱,按90%的相似度水平可分为2个群,其它菌株的相似性较低。结论:余杭区存在遗传谱系密切相关的副溶血弧菌流行克隆。

Objective：To apply pulsed-field gel electrophoresis（PFGE） to investigating genotype of Vibrio parahaemolyticus strains isolated from food poisoning,and to determine relationship among the strains.Methods： Chromosomal DNAs from 19 isolates in agarose were digested with the restriction enzyme Not 1,and then were analyzed by pulsed field gel electrophoresis.Using Bio Numerics software clustered PFGE patterns.Results： There were 9 distractive PFGE patterns.Taking 90% similitude as criterion,these strains were divided into 2 clusters.Conclusion： There were closely related epidemic clones of Vibrio parahaemolyticus in Yuhang.