24h内不同俯/仰卧位通气时间比对急性肺损伤模型病理的影响

Effect of different prone time/supine lime ratios to pathology of mini-pig with acute lung injury

目的比较24h内不同俯“印卧位辅助通气时间比对小型猪急性肺损伤（ALI）病理结果的影响。方法选取7曲个月龄健康雌性小型猪15只，平均体质量（30．2-3．2）kg，身长（76．5±5．9）cm。麻醉后行气管插管机械通气，经气管插管注射pH1．0盐酸制备呼吸窘迫综合征（ARDS）模型。随机分为8h组（俯卧8h±仰卧16h组），16h组（俯卧16h±仰卧8h组），24h组（持续俯卧24h组）各5只小型猪。24h后对模型动物实施安乐死，取出肺组织观察大体标本，计算肺湿干重比（W，D）。肺组织甲醛固定，苏木素-伊红（HE）染色切片，参照半定量评分标准进行评分。结果3组间肺炎评分与W／D比较，16h组与24h组均低于8h组，但差异不显著。结论早期足量（16h或以上）的俯卧位辅助通气可能减少急性肺损伤动物模型的肺部炎性反应。

Objective To compare the effects of different prone and supine position duration within 24-hour period on mini-pig acute lung injury （ALI）models. Methods Fifteen female mini-pigs,mean weight （30.2±3.2） kg, length （76.5±5.9） cm were anesthetized and ventilated. Hydrochloric acid （pH 1.0） was aspirated intratracheally to create ALI model. Mini-pigs were assigned randomly into three groups： p rone 8 h group, prone 8h then supine 16 h; 16 h group, prone 16 h then supine 8 h; 24 h group, prone 24 h continuously. Min-pigs were euthanasia at the end of the experiment to take out the lung, examine the tissue change and calculate the wet to dry weight ratio of lung （W/D）. Sample was cut into slices and carried on hematoxylin and eosin （HE） stain which was intended to calculate injury, score by semi ration score criteria. Results W/D, and injury score were lower in 16 h group and 24 h group than the 8 h group, whereas no significant differences among the three groups. Conclusions Prone position for 16 hours or longer could decrease oxygenation by decreasing inflammation in acute lung injury（ALI ）animal models.