正常和异常血清的共振散射光谱

Resonance Scattering Spectra of Normal Serums and Abnormal Serums

用日本岛津RF-5301荧光分光光度计研究了高甘油三脂、高胆固醇、高血糖血清和正常血清的散射光谱及同一生化指标不同浓度血清散射光谱的规律。实验结果表明,正常血清散射光谱与高血脂和高血糖血清散射光谱的特征峰差异明显,可以据此区分正常和高血糖、高血脂血清;同一生化指标不同浓度血清散射光谱的峰值强度随血脂、血糖浓度的增大而增强,峰值强度I446/I464和I615/I628的值随血糖浓度的增大近似呈线性变化,而高血脂血清的散射光谱则无此现象,据此可区分高血糖和高血脂血清。此研究为临床医学应用提供了可靠的实验依据。

The scattering spectra of high triglycerides, high cholesterol, high blood sugar and normal serum were researched with Shimadzu RF-5301 fluorescence spectrophotometer, and the scattering spectra of serums with same biochemical parameters. The scattering spectra of abnormal serums, such as the serums with hyperlipidemia and the serums with hyperglycemia, have obvious differences from the spectra of normal serums at the characteristic peaks. This result has laid the foundation of distinguishing between normal and high blood sugar, high blood lipids serum. The peak intensity of scattering spectra in the same biochemical markers of different concentrations serum increased with the blood lipids, blood glucose concentrations. Moreover, the peak intensity ratio of I446/I464 and I615/I628 increased with blood sugar concentration, and has a nearly linear relationship. However, there is no similar phenomenon in the scattering spectra of serums with hyperlipidemia. Hence, it can be used to distinguish between the serums with hyperglycemia and the serums with hyperlipidemia. This study provides an experimental basis for clinical application.