摘要
目的:测定异甾体生物碱( 鄂贝啶碱ebeiedine、去氢鄂贝啶碱ebeiedinone、浙贝甲素verticine、异浙贝甲素isoverticine、浙贝乙素verticinone 和西贝素imperialine) 在贝母属(Fritillaria)16 种植物鳞茎中的含量,探讨贝母所含生物碱与中医临床应用的关系。方法:样品经三甲基硅烷咪唑(TMSI) 柱前衍生化后,进行毛细管气相色谱分析。结果:根据所测生物碱在各种贝母中存在与否及生物碱结构类型,将所分析的16 种贝母分为4 类。结论:不同种贝母所含的生物碱种类与量有差异,由于生物碱是贝母类中药的主要有效成分,因而本文为中医临床区别应用各种贝母( 如浙贝母,川贝母) 提供了科学依据。
AIM: To determine the contents and investigate the existence of isosteroidal alkaloids (ebeiedine, ebeiedinone, verticine, isoverticine, verticinone and imperialine) in 16 different Fritillaria species. METHODS: Samples were pre derivatized by trimethylsilylimidazole, and analyzed using gas chromatography. RESULTS: Sixteen different Fritillaria species studied in present paper were classified into 4 groups according to the existence and the structure type of the alkaloids determined in each species; (1) Only containing those 5α cevenine isosteroidal alkaloids (verticine, verticinone, ebeiedine etc.) whose hydrogens of C 13 and C 17 being at the state of trans configuration (e.g. F.thunbergii, F.hupehensis etc.); (2) Only containing those alkaloids (imperialine) whose hydrogens of C 13 and C 17 being at the state of cis configuration (e.g. F.pallidiflora ); (3) Containing both types of alkaloids (verticine, verticinone and imperialine etc.) whose hydrogens of C 13 and C 17 being at the state of trans or cis configuration (e.g. F.cirrhosa ); and (4) No alkaloid mentioned above was detected at the present conditions (e.g. F.meleagroides ). CONCLUSION: The resnets showed that the type and content of isosteroidal alkaloid varied in different Fritillaria species. This provided scientific evidence for the clinical use of these crude drug Beimus.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第11期842-847,共6页
Acta Pharmaceutica Sinica