己酮可可碱对创伤性急性肺损伤兔肺组织肿瘤坏死因子-α基因表达的影响及其意义

Effect and significance of pentoxifylline on pulmonary tumor necrosis factorα mRNA expression in rabbits with traumatic acute lung injury

目的：观察己酮可可碱（ＰＴＸ）对创伤性急性肺损伤（ＡＬＩ）家兔肺组织肿瘤坏死因子 α基因（ＴＮＦ αｍ ＲＮＡ）表达的影响，探讨ＰＴＸ在创伤性ＡＬＩ治疗中的作用机制。方法：采用创伤性ＡＬＩ模型，实验动物随机分为正常对照组（８ 只）、创伤性ＡＬＩ模型组（２４ 只）和ＰＴＸ治疗组（２４ 只），用ＥＬＩＳＡ 和逆转录聚合酶链反应（ＲＴ ＰＣＲ）方法分别检测不同时间点血浆ＴＮＦ α含量及肺组织ＴＮＦ αｍ ＲＮＡ表达水平。结果：血浆ＴＮＦ α含量创伤性ＡＬＩ模型组于创伤后１．５ 小时达峰值〔（１．２７±０．２６）μｇ／Ｌ〕，肺组织ＴＮＦ αｍ ＲＮＡ表达于创伤后８ 小时达高峰（２．６７±０．１８），二者在时间上不完全平行。ＰＴＸ治疗组肺组织ＴＮＦ αｍ ＲＮＡ 的表达和外周血ＴＮＦ α含量均低于创伤性ＡＬＩ模型组（Ｐ均＜ ０．０５）。结论：ＰＴＸ能有效抑制创伤性ＡＬＩ家兔肺组织ＴＮＦ αｍ ＲＮＡ表达；本研究为ＰＴＸ治疗创伤性ＡＬＩ提供了实验依据。

Objective:To investigate the effects of pentoxifylline (PTX) on pulmonary expression of tumor necrosis factorα (TNFα) in rabbits with acute lung injury (ALI),and explore the mechanism underlying PTX on the treatment of ALI.Methods:Traumatic ALI models were used in this study.Rabbits were randomly divided into three groups as follows:normal control group ( n =8),ALI model group ( n =24),and PTX treatment group ( n =24).ELISA and reversetranscription polymerase chain reaction methods were used to detect the TNFα contents in plasma and TNFα mRNA levels in lung tissues at various intervals.Results:Peak value of TNFα in plasma was found at 1 5 hours after trauma 〔(1 27±0 26)μg/L〕,while the peak value of TNFα mRNA in lung tissues was presented at 8 hours after trauma (2 67±0 18),no parallel pattern was found between plasma TNFα and pulmonary TNFα mRNA levels.Treatment with PTX could markedly inhibit the expression of pulmonary TNFα mRNA and decrease plasma TNFα contents following traumatic ALI (both P <0 05).Conclusions:PTX can effectively inhibit the expression of pulmonary TNFα mRNA in rabbits with traumatic ALI.The present study may provide the experimental basis for use of PTX in treating traumatic ALI.