缺氧和营养不良对大鼠膈肌功能肌电图的影响及复方红景天对膈肌功能保护作用的观察

The effects of hypoxia and malnutrition on diaphragm electromyography and protective effect of Fufang Hongjingtian(复方红景天) on diaphragmatic function in rats

目的：探讨慢性缺氧和营养不良对膈肌功能和膈肌肌电图（ＥＭＧｄｉ）的影响以及复方红景天对膈肌功能的保护作用。方法：将３２ 只Ｗｉｓｔａｒ大鼠随机分为慢性缺氧组（放入常压缺氧箱内，氧浓度０．１１０±０．００５，每日８ 小时，Ａ 组）、营养不良组（每日进正常食量的１／２，Ｂ组）、缺氧加复方红景天组（缺氧方法同Ａ组，每日缺氧前灌服复方红景天１．０ ｇ／ｋｇ，Ｃ组）和正常对照组（Ｄ组），每组８只。实验５ 周在麻醉下测体重、ＥＭＧｄｉ和膈肌功能；动物处死后测膈重。结果：Ｂ组体重低于Ａ、Ｃ、Ｄ组５０％ ，膈肌显著变薄，面积缩小，重量减轻；Ａ、Ｂ组ＥＭＧｄｉ的高频份量与低频份量比值（Ｈ／Ｌ）、中心频率（Ｆｃ）和电刺激时膈肌最大收缩力、最大强直收缩、疲劳指数均较Ｃ、Ｄ组显著降低；强直收缩时间和疲劳时间较Ｃ、Ｄ组显著缩短，Ａ组与Ｃ、Ｄ组，Ｂ组与Ｃ、Ｄ组之间比较均有非常显著性差异（Ｐ均＜ ０．０１）；Ａ组与Ｂ组、Ｃ组与Ｄ组比较则无显著性差异。结论：慢性缺氧和营养不良能明显引起膈肌疲劳；复方红景天对缺氧性膈肌疲劳具有明显保护作用。

Objective:To study the influence of chronic hypoxia and malnutrition on diaphragmatic fatigue (DF) with and the protective effect of Fufang Hongjing(复方红景天) tian on diaphragmatic function in rats.Methods:32 Wistar rats were randomly divided into four groups,group A with chronic hypoxia (exposed to low oxygen content of 0 110±0 005,8 hours/day),group B with malnutrition(feeding 1/2 forage of normal quantity per day),group C with chronic hypoxia and Hongjingtian (hypo Xia combined with lavage of Fufang Hongjingtian 1 0 g·kg -1 ·day -1 ),and group D as normal controls.The diaphragm electromyography (EMGdi),were measured in 32 rats after 5 weeks.Results:H/L and Fc of EMGdi,maximal contraction,tetanic contraction,tetanic contraction time,contraction index and fatigue index of diaphragm in group A and group B were significantly lower than those in group C and group D,and there were also significantly differences between group A and group C or D,group B and group C or D (all P <0 01).while no marked differences were observed between group A and B,group C and D,respectively.Conclusions:Chronic hypoxia and malnutrition can significantly cause DF,and Fufang Hongjingtian has protective effect on hypoxic DF.