地塞米松对肺损伤大鼠肺泡巨噬细胞释放肿瘤坏死因子-α和白细胞介素-6的影响

Effects of dexamethasone on the release of tumor necrosis factorα and interleukin6 from alveolar macrophages in rats with lung injury

目的：观察博莱霉素（ＢＬＭ）诱发大鼠肺损伤后肺泡巨噬细胞（ＡＭ）释放肿瘤坏死因子 α（ＴＮＦ α）和白细胞介素 ６（ＩＬ ６）的变化及地塞米松（ＤＭ）对ＡＭ 释放ＴＮＦ α和ＩＬ ６的影响。方法：采用生物活性法测定ＴＮＦ α和ＩＬ ６。结果：灌注ＢＬＭ 后ＡＭ 释放ＴＮＦ α〔（５８．９５±１３．２１）ｋＵ／Ｌ〕和ＩＬ ６〔（３５．００±１０．００）ｋＵ／Ｌ〕明显高于对照组〔ＴＮＦ α为（２３．６３±５．５０）ｋＵ／Ｌ，Ｐ＜ ０．００１；ＩＬ ６ 为（２１．１７±６．０４）ｋＵ／Ｌ，Ｐ＜０．０１〕，ＤＭ 能够抑制ＡＭ 释放ＴＮＦ α和ＩＬ ６，并且与其剂量相关（随ＤＭ 浓度的增加，ＡＭ 分泌ＴＮＦ α和ＩＬ ６ 水平逐渐降低）。ＢＬＭ 组支气管肺泡灌洗液（ＢＡＬＦ）中细胞总数（４．８８±０．６５）×１０６ 也明显高于对照组〔（１．４７±０．４１）×１０６，Ｐ＜ ０．００１〕。结论：ＤＭ 能以剂量依赖方式抑制ＡＭ 释放ＴＮＦ α和ＩＬ

Objective:To observe the changes of tumor necrosis factorα(TNFα) and interleukin6(IL6) released by alveolar macrophages (AM) and the effect of dexamethasone (DM) on TNFα and IL6 levels in bleomycininduced lung injury in rats.Methods:TNFα and IL6 levels were measured by bioassay methods.Results:Increased releases of both TNFα〔(58 95±13 21)kU/L vs.(23 63±5 50)kU/L, P <0 001〕 and IL6 〔(35 00±10 00)kU/L vs.(21 17±6 04)kU/L, P <0 01〕 by AM were noted after bleomycin administration compared with controls.However,treatment with DM markedly inhibited releases of TNFα and IL6 by AM in a dosedependent manner.In addition,total cell counts in bronchoalveolar lavage fluid were much higher in bleomycin group than that in the controls 〔(4 88±0 65)×10 6 vs.(1 47±0 41)×10 6, P <0 001〕.Conclusions:These results indicate that DM can markedly inhibit releases of TNFα and IL6 by AM in a dosedependent manner.