恶性疟原虫中国海南株裂殖子表面蛋白1 基因的序列分析(英文)

SEQUENCE ANALYSIS OF THE MSP 1 GENE OF PLASMODIUM FALCIPARUM ISOLATES FROM HAINAN, CHINA

目的:测定恶性疟原虫中国海南分离株MSP1 基因的全序列,并进行多态性分析。方法:来自海南省保亭县2 例恶性疟患者的血样(分别滴在滤纸上)直接制备基因组DNA 后,用MSP1 基因特异的5 对寡核苷酸引物在体外扩增目的基因,用ABIPRISMTM末端标记循环测序试剂盒进行直接测序,并将测序结果与已知的等位基因型进行比较分析。结果:获得了2 个恶性疟原虫中国分离株MSP1 基因的全序列,与已知的MSP1 等位基因型进行序列比较,证实其属于MAD20 型。推导其氨基酸序列除了第2 区、第4 区和第8 区以外,其余序列完全一致。其中HN2 的第4 区含K1 型序列。结论:两株恶性疟原虫海南株的MSP1 基因属于MAD20 型,与MAD20 等位基因比较,其推导的氨基酸序列存在一些小差异。本文首次提供了恶性疟原虫中国(海南)分离株MSP1

AIM: To obtain the complete sequence and analyze the diversity of the MSP 1 molecule from the Chinese isolates of Plasmodium falciparum . METHODS: Genomic DNA was prepared directly from blood samples spotted on filter papers from 2 malaria patients from Baoting County, Hainan Province. PCR amplification of the target gene was carried out using 5 pairs of oligonucleotides specific for the MSP 1 gene. Direct sequencing of the target gene fragments was performed using ABI PRISM TM Dye Terminator Cycle Sequencing Ready Reaction Kit(Perkin Elmer) in a automatic ABI PRISM TM 310 Genetic Analyzer. RESULTS: For the first time, two complete sequences of the MSP1 gene from two Chinese isolates of Plasmodium falciparum were obtained. A comparison with the previously reported sequences identified them as members of the MAD20 allelic family. The deduced amino acid sequences of the MSP1 from this two Chinese isolates were identical with each other except for Blocks 2, 4 and 8. CONCLUSION: The sequences of the MSP 1 from two Chinese isolates of P. falciparum belong to the MAD20 allelic family. Minor variations through the whole sequences exist compared with the MAD20 sequence. The results provide the first evidence of the diversity of the MSP 1 molecule from Chinese isolates of Plasmodium falciparum .