表皮葡萄球菌在慢性鼻-鼻窦炎患者中生物膜的表达

The expression of Staphylococcus epidermidis biofilm in patients with chronic rhinosinusitis

目的研究表皮葡萄球菌生物膜在慢性鼻-鼻窦炎(CRS)患者鼻黏膜中的表达情况。方法将临床手术中获取的180例慢性鼻-鼻窦炎患者内镜手术黏膜样本,根据细菌培养结果将其分为4组,分别为表皮葡萄球菌生长组79例,其他葡萄球菌生长组33例,阴性杆菌生长组27例,细菌培养阴性组41例,所有样本均行标准的扫描电子显微镜方法检测。结果表皮葡萄球菌生长组79例全部观察到生物膜的存在,表达率为100%(79/79),其他葡萄球菌生长组为75.8%(25/33),阴性杆菌生长组为77.8%(21/27),细菌培养阴性组为41.5%(17/41)。后3组分别与表皮葡萄球菌组比较,P值均<0.01。结论表皮葡萄球菌在CRS患者生物膜形成中起重要作用。

Objective To investigate the expression of Staphylococcus epidermidis biofilm in the nasal mucosa of patients with chronic rhinosinusitis（CRS）.Methods 180 samples were collected during endoscopic sinus surgery from patients with CRS.According to the results of bacterial culture,they were classified into 4 groups [Staphylococcus epidermidis growth group（79 cases）,other Staphylococcus aureus growth group（33 cases）,negative bacillus growth group（27 cases） and bacterial culture-negative group（41 cases）].All samples were subjected to scanning electron microscopy.Results The scanning electron microscopy analysis showed bacterial biofilms in all cases of the Staphylococcus epidermidis growth group,and the expressions for the 4 groups were 100%（79/79）,75.8%（25/33）,77.8%（21/27） and 41.5%（17/41）,respectively.The results were compared between Staphylococcus epidermidis growth group and other 3 groups（P0.01）.Conclusions Staphylococcus epidermidis is important for the formation of biofilms in the patients with CRS.