柯萨奇B_3病毒致小鼠慢性心肌炎的初步研究

A preliminary study on chronic viral myocarditis in mice

目的 探讨在建立慢性病毒性心肌炎及扩张型心肌病动物模型（ＤＭＣ） 的实验途径，获得制造该模型所需的必要实验数据。方法 对柯萨奇Ｂ３ 亲心肌株病毒（ＣＶＢ３ｍ） 进行驯化，感染不同品系的纯系实验小鼠。观察并记录感染小鼠的死亡及发病情况。结果 实验组昆明鼠和ＮＩＨ 小鼠的死亡率分别为２－５％ 和７－５％ ， 二者的心肌未见有明显的病理改变；Ｂａｌｂ／ｃ 雄鼠感染ＣＶＢ３ｍ 后死亡率为５１－７％ ，且发病较重；Ｂａｌｂ／ｃ 雌鼠感染经驯化病毒后， 实验组小鼠死亡率为２７－８％ ， 感染病毒后１０～２０ 天心肌组织均有不同的病理改变，４０ 天后实验组小鼠心脏与体重的比值明显大于对照组。结论 Ｂａｌｂ／ｃ 雌鼠感染经驯化的ＣＶＢ３ｍ 为建立慢性病毒性心肌炎及扩张型心肌病动物模型提供了一个实验途径。

Objective To investigate the feasibility and features of animal models of chronic viral myocarditis and dilated cardiomyopathy Methods Different kinds of bred mice were infected with Coxsackie virus B 3 (CVB 3m ), which was tamed in Hela and cultured mouse myocardiocytes Mortalities and pathological changes were observed and analyzed Results In experiment groups which were infected by CVB 3m , the death rates of Kunming and NIH mice were 2 5% and 7 5%, respectively. No significant pathological changes were identified The Balb/c mice had a higher death rates of 51 7% and 27 8% in male and female mice, respectively. Significant pathological changes were found from 10 to 20 days after being infected with CVB 3m The ratio of heart and body weight of the female Balb/c mice was significantly higher in infected mice than that of contrels Conclusion Female Balb/c mice infected with tamed CVB 3m might be used as a murine model of chronic viral myocarditis and dilated cardiomyopathy