上海人幽门螺杆菌vac A基因表型与相关胃肠疾病

The genotype of vac A gene of Helicobacter pylori (Hp) and its correlation with the gastroduodenal diseases associated with Hp

目的 探讨用ｖａｃ Ａ 基因表型检测对幽门螺杆菌（Ｈｐ） 进行分型，并分析不同基因型与相关胃肠疾病之间的关系。方法 对临床胃黏膜标本中Ｈｐ 菌株进行分离培养及其总ＲＮＡ 抽提，逆转录聚合酶链反应检测ｖａｃ Ａ基因表型。结果 ５０ 株临床分离Ｈｐ 菌株，ｖａｃ Ａ 基因表型ｓ１／ｍ２ 型４６株（９２％ ），ｓ１／ｍ１ 型４ 株（８ ％） ，未发现ｓ２ 型。结论 上海人Ｈｐ 菌株ｖａｃ Ａ 基因绝大多数表达ｓ１／ｍ２型。ｓ１／ｍ２ 基因表型分布于所有Ｈｐ 相关胃十二指肠疾病的Ｈｐ 菌株中。

Objective To analyze the expression of vac A gene of Helicobacter pylori (Hp) isolated from Chinese patients and to find out the relationship between the genotype of Hp vac A and the gastroduodenal diseases associated with Hp. Methods Clones of Hp obtained from patients with gastroduodenal diseases were cultured and mRNA extraction was performed with reverse transcriptase polymerase chain reaction (RT PCR). Results 92% of the strains isolated in Shanghai(China) was found to be s1/m2. Strains expressing s1/m1 forms were obtained in 4 cases. s2 form was not found. Conclusion The overwhelming majority of strains isolated from Shanghai patients expresses s1/m2 from. The s1/m2 genotype is dispersed in all of the gastroduodenal diseases associated with Hp.