聚合酶链反应检测外周血和腹水标本中结核菌的价值

Evaluation of detection of M.tuberculosis in peripheral blood and ascites samples by polymerase chain reaction in tuberculous peritonits

目的探讨聚合酶链反应（PCR）技术检测外周血白细胞和腹水中结核分支杆菌在结核性腹膜炎诊断中的价值。方法应用RCR和抗酸染色镜检（镜检）对58例结核性腹膜炎和30例对照组外周血白细胞及腹水标本进行结核菌检测。结果58例结核性腹膜炎外周血和腹水RCR阳性率分别为79．3％和60．3％，外周血、腹水标本配对检测总阳性率为88％。腹水镜检阳性率为1．7％，PCR与镜检比较，x2检验，P＜0．001，差异有显著性。结论PCR检测外周血和腹水标本中结核菌DNA具快速、简便、灵敏及特异等优点，对结核性腹膜炎的诊断和鉴别诊断有重要价值，两者联合检测可提高敏感性和准确性。

Objective To evaluate clinical value of detection of M. tuberculosis in peripheral blood and ascitessamples by polymeras chain reaction (PCR) technique in diagnosis of tuberculous peritonitis.Methots PCRand standard light -microsoopy were used to detect M. tuberculosis in peripheral blood and ascites samplesobtained from 58 patients with tuberculous peritonitis and 30 patients without tuberculous peritonitis.ResultsIn blood and ascites samples of 58 patients with tuberculous peritonitis,the positive rate of PCR was 79. 3%and 60. 3% respectively,tolal positive rate of combinative detevtin of blood and ascites samples was 88%, inascites samples the positive rate was 1. 7 % in standard light -microscopy. A statistically obvious difference wasseen (P<0.001). Conclusions PCR is rapid,Simple,specific and sensitive for detection of M.tuberculosisin blood and ascites smnples.and it is of great value in the diagnosis of tuberculous peritonitis and differentiation of tuberculous peritonitis from ther peritonsistis.