β-Catenin信号转导通路在肺癌A549细胞顺铂耐药中的作用

The Role of β-Catenin Pathway on DDP Resistance in Human Lung Cancer A549 Cell

目的建立肺癌A549顺铂多药耐药细胞株,研究肺癌耐药的机制。方法使用顺铂逐步增加剂量和间歇大剂量冲击相结合的方法诱导肺癌A549细胞,以建立其多药耐药细胞株A549/DDP;MTT细胞毒实验比较A549与A549/DDP细胞对顺铂的敏感性;绘制A549和A549/DDP细胞生长曲线,观察二者的增殖速度变化;Western blotting实验显示A549和A549/DDP细胞之间Akt、p-Akt、GSK-3β、p-GSK-3β和β-Catenin蛋白的表达改变。结果经过30周的诱导我们建立了肺癌A549细胞的顺铂耐药细胞株A549/DDP;MTT细胞毒实验结果显示顺铂对A549和A549/DDP细胞的IC50值分别为(1.37±0.09)和(11.63±0.74)μmol/L,与A549细胞比较,A549/DDP细胞对顺铂耐药8.47倍,生长曲线结果显示A549/DDP细胞的增殖速度与A549细胞没有显著性差异;Western blotting的结果显示A549/DDP细胞的Akt磷酸化水平升高,抑制了下游GSK-3β的活性,导致了细胞内β-Catenin蛋白的上调。结论建立了肺癌A549顺铂多药耐药细胞系A549/DDP,A549/DDP细胞中β-Catenin通路的激活与其耐药性的形成有一定的相关性。

Objective To establish a cisplatin （DDP） resistance A549 cell line and to explore the mechanisms of multidrng resistance in human lung cancer. Methods The human cancer A549 cells were exposed in gradually increasing or interval high dose of DDP. MTr assay was used to detect the cytotoxic activity of DDP against A549 and A549/DDP cells. Cell numbers were calculated to draw growth curve. The protein expressions of Akt, p -Akt, GSK -3β, p- GSK - 3β and β -Catenin were determined by western blotting analysis in A549 and A549/DDP cells. Results DDP resistance cell line AS49/DDP was established by DDP continuous iriducing. The IC50 values of DDP against A549 and A549/DDP cells were （ 1.37 ± 0. 09 ） and （ 11.63 ± 0. 74 ） μmol/L by MTI7 assay respectively. The cells growth curve showed that cell proliferation had no significant difference between A549 and A549/DDP cells. Western blotting analysis showed that phospho - Akt was up - regulated in A549/DDP cells, which elevated the phosphorylation of GSK - 3β at sefine 9. Compared to A549 cells, the up - regulation of β - Catenin protein was found in A549/DDP cells. Conclusions A549/DDP showed resistant to DDP and was a reliable muhidrug resistance cell model. The β - Catenin pathway played an important role in the resistant phenotype of A549/DDP cell line.