番茄红素对肺癌患者围术期免疫功能的调节

The Lycopene's Modulation toImmune Function of Lung Cancer Patients

目的观察番茄红素预处理后肺癌患者T细胞亚群及NK细胞的变化,探讨其对肿瘤患者免疫功能的影响。方法 30例肺癌拟行肺叶切除的患者分为两组(n=15)。实验组(组Ⅱ)术前1周开始,每天1次服用6%的番茄红素4 g,对照组(组Ⅰ)给予安慰剂,实验前(T1)、术前(T2)和术后第1(T3)、3(T4)、7(T5)天取外周静脉血1.5 mL,流式细胞术测定T细胞亚群和NK细胞。结果 T细胞亚群:组Ⅰ从T2开始,CD 3+、CD 4+和(CD 3++CD 4+)/(CD 3++CD 8+)显著下降(P<0.05),T3、T4下降更明显(P<0.01),至T5时仍低于T1时(P<0.05);组Ⅱ在T3、T4时CD3+、CD4+和(CD3++CD4+)/(CD3++CD8+)值显著降低(P<0.05),T5与T1时比较无显著差异(P>0.05),两组T2、T5时比较差异性显著(P<0.05);NK细胞:组Ⅰ在T2、T3时显著降低(P<0.05),T4、T5时降低更为明显(P<0.01)。组Ⅱ在T2、T3及T4时显著降低(P<0.05),T5与T1时比较无显著差异(P>0.05),两组在T4、T5时比较差异显著(P<0.05)。结论番茄红素预处理可以明显减轻肺癌患者围术期免疫功能的抑制,促进免疫功能的恢复。

Objective To observe the variation of T - lymphocyte subsets and natural killer cells of lung cancer patients after pretreatment with the use of lyeopene, investigate the effects of it on immune function of lung cancer patients. Methods 30 lung cancer patients planning to do pulmonary lobectomy were divided into two groups （n = 15）. One week before operation, the patients in experimental group （group 11 ） took 6% lycopene orally everyday, 4 g per day, the patients in control group （ group Ⅰ ） took placebo orally everyday. On pre - experiment （ T1 ） , preoperative （ T2 ） and the first （T3 ） , third （T4） , seventh （T5 ） day, peripheral venous blood samples were taken to determinate T - lymphocyte subsets and NK cell with the use of flow eytometer. Results T - lymphocyte subsets ： In group Ⅰ , CD3, CD4＋ and （ CD3 ＋ CD4＋ ） / （ CD3 ＋ CD8 ） decreased significantly from T2 （ P 〈 0. 05 ）, decreased more significantly in T3, T4 （ P 〈 0.01）, were still lower than that of T1 whenW5 （P〈0.05）. In group Ⅱ, CD3, CD4 and （CD3 ＋CD4） / （CD3 ＋ CD8 ） decreased significantly at T3 and T4 （P 〈 0. 05 ） , but had no significant difference in T5 compared with T1 （P 〉 0. 05 ） , the difference between two groups were significant at T2 and T5 （ P 〈 0. 05 ）. NK cell ： In group Ⅰ , it decreased significantly in T3, T3 （P 〈0. 05） and more significantly in T4 , T5 （P 〈0. 01 ）. In group 11 , it decreased significantly in T2 , T3 and W4 （P 〈 0.05） , but had no significant difference at T5 compared with T1 （ P 〉 0. 05） , the difference between the two groups were significant in T4 and T5 （ P 〈 0. 05 ）. Conclusions Pretreatment with lyeopene can relieve immunosuppression of lung cancer patients in Peroperative Period significantly and hasten the recovery of immune function.