气相色谱-三重串联四极杆质谱联用测定食用油中残留的腐霉利

Determination of Procymidone Residues in the Edible Vegetable Oils Gas Chromatography-triple Quadrupole Mass Spectrometry

为了快速检测和确证食用油中的腐霉利残留量,建立了气相色谱-三重串联四极杆质谱联用法(GC-MS/MS)测定食用油中的腐霉利残留分析方法。样品用(V/V=1:1)环己烷/乙酸乙酯溶解,凝胶渗透色谱(GPC)净化浓缩后,采用GC-MS/MS多反应监测(MRM)模式检测,外标法定量。腐霉利为1.0-200.0 mg/L浓度范围内与其峰面积呈线性关系,其相关系数(R)为0.9999。在食用油中的腐霉利残留检限为5.0 mg/kg,添加回收率在为75.6%～105.7%之间。

For validation and fast determination of procymidone residue in the edible vegetable oils,the analytical method was established to determine procymidone in the edible vegetable oils by gas chromatography-triple quadrupole mass spectrometry（GC-MS/MS）.The edible vegetable oils were dissolved by cyclohexane and ethyl acetate（V/V=1：1）,and cleaned up by gel permeation chromatography.Qualitative analysis and quantitative determination were carried out by GC-MS/MS in multiple reaction monitoring（MRM） mode.There was good linear relationship between the peak area and concentration of procymidone in the range from 1.0mg /L to 200.0 mg /L,and the correlation coefficient（R） was 0.9999.The limit of detection of procymidone in the edible vegetable oils was 5.0 mg/kg,and the recovery yields of procymidone were 75.6%-105.7%.