Skp2和p27在乳腺癌分子亚型中的表达及与基底样型乳腺癌的关系

Expression of Skp2 and p27 in molecular subtypes of breast carcinoma and their relation with basal-like breast carcinoma

目的探讨Skp2和p27在乳腺癌分子亚型中的表达及与基底样型乳腺癌的相关性。方法 1 452例乳腺癌标本制作组织芯片,免疫组化MaxVision快捷法检测ER、PR、HER2、CK5/6和EGFR,按照Nielsen标准进行分子分型。同时检测Skp2和p27。结果在乳腺癌基底样型、HER2过表达、裸表达型、腺腔A型、腺腔B型中,Skp2表达率为63.3%、51.9%、53.1%、21.3%和13.7%;p27表达率为35.2%、46.3%、41.4%、55.9%和63.0%。Skp2在基底样型、HER2过表达型和裸表达型的表达中显著高于腺腔A和B型(P<0.01);而p27在基底样型、HER2过表达型和裸表达型中的表达率显著低于腺腔A和B型(P<0.05)。Skp2和p27的表达水平与ER表达相关(P<0.01)。Skp2和p27的表达在基底样型乳腺癌中呈负相关(P<0.01)。结论 Skp2的高表达及p27的失表达可能与ER阴性乳腺癌的发生和发展有关,并且Skp2在基底样型乳腺癌的发生、发展中起重要作用。在基底样型乳腺癌中二者基因相关性的研究为其发病机制的阐明提供帮助。

Objective To study the expression of Skp2 and p27 in the molecular subtypes of breast carcinoma and their correlation with basal-like breast carcinoma.Methods Immunohistochemistry was used to detect the expression of ER,PR,HER2,CK5 /6 and EGFR on a tissue microarray containing 1 452 cases of invasive breast carcinomas.Based on the results,the cases were categorized according to Nielsen criteria.Immunohistochemical MaxVision method was also used to detect the expression of Skp2 and p27 on a tissue microarray.Results Positive expression rates of Skp2 protein in basal-like,HER2 over-expression,null,luminal A and luminal B substypes were 63.3%,51.9%,53.1%,21.3% and 13.7%,respectively;the expression rates of p27 protein were 35.2%,46.3%,41.4%,55.9% and 63.0%,respectively.Like the phenotypes of basal-like,HER2 over-expression and null,positive expression of Skp2 protein were remarkably higher than luminal A subtype and luminal B subtype（P〈0.01）;positive expression of p27 protein were remarkably lower than luminal A subtype and luminal B subtype（P〈0.05）.The expression of Skp2 protein and p27 protein was associated with the expression of ER protein（P〈0.01）.Expression of Skp2 was negatively correlated with expression of p27 in basal-like breast carcinoma（P 0.001）.Conclusion The over-expression of Skp2 protein and loss of p27 expression may be related to the development of ER-negative breast cancer.Skp2 might play important roles in the development of basal-like breast carcinoma.The research of the relationship between Skp2 and p27 in basal-like breast carcinoma may help for illuminating the pathogenesis.