40例糖尿病患者血栓形成前期若干相关指标的变化

STUDY OF SOME INDEXES RELATED TO PRETHROMBOTIC PHASE IN DIABETICS

目的： 观察糖尿病患者血栓形成前期血浆内皮素１ （ＥＴ１） 、血小板α－ 颗粒膜蛋白（ＧＭＰ１４０） 、组织纤溶酶原激活物（ｔＰＡ） 及其抑制物（ＰＡＩ１） 的变化。方法： 分别采用放射免疫法和发色底物显色法， 检测了２０ 例正常人和４０ 例Ⅱ型糖尿病患者（２２ 例无微血管病变、１８ 例有微血管病变） 的ＥＴ１ 、ＧＭＰ１４０ 、ｔＰＡ 及ＰＡＩ１ 的变化。结果： 糖尿病患者的ＥＴ１ 、ＧＭＰ１４０ 、ＰＡＩ１ 显著高于正常对照组， ｔＰＡ 则明显下降， 伴微血管病变者比无微血管病变者变化更明显， 且ＥＴ１ 与ＧＭＰ１４０ 、ＰＡＩ１ 呈显著正相关， 而与ｔＰＡ 呈显著负相关。

Objective:To observe changes of the plasma endothelin 1(ET 1),platelet α granule membrane protein(GMP 140 ),tissue type plasminogen activator(tPA) and plasminogen activator inhibitor(PAI 1)in diabetics.Methods:ET 1,GMP 140 ,tPA and PAI 1 were measured by using radioimmunoassy and developing substrate method respectively in 40 patients with type Ⅱ diabetes mellitus(18 patients with microangiopathy,22 without microangiopathy) and in the 20 controls.Results:ET 1,GMP 140 and PAI 1 were significantly higher in the diabetic patients than those in the controls,whereas tPA was obviously lower.The changes of the above indexes were more apparent in patients with microangiopathy than those without.There was significantly positive correlation between plasma ET 1 and GMP 140 ,PAI 1,whereas there was obviously negative correlation between ET 1 and tPA.Conclusion:The measurement of the above some indexes related to prethrombotic phase has an important value in the early diagnosis and analysis of diabetic microangiopathy.