四藤方对人胃癌SGC-7901细胞黏附和侵袭的影响

Treatment with Sitengfang decreases cell adhesion,invasion and migration in human gastric carcinoma cell line SGC-7901

目的:观察四藤方对胃癌细胞SGC-7901黏附、侵袭、移动等转移生物学行为的影响.方法:体外培养SGC-7901细胞,分为四藤方50、100、200mg/L组和对照组,采用Cytoselect48-Well Cell Adhesion Assay检测胃癌细胞与基质黏附能力,利用Transwell侵袭转移模型评价四藤方对胃癌细胞侵袭能力的影响,划痕实验观察四藤方对SGC-7901细胞移动能力的影响.结果:终浓度50、100、200mg/L四藤方能显著降低SGC-7901细胞黏附能力(0.34±0.05,0.13±0.00,0.12±0.01vs1.34±0.12,P<0.01),抑制SGC-7901细胞侵袭(39±3,26±6,8±2vs80±5,P<0.01),终浓度50mg/L四藤方作用48h可显著抑制SGC-7901细胞迁移(28.57%±4.10%vs59.68%±5.98%,P<0.01).结论:四藤方可降低SGC-7901细胞的黏附能力,侵袭能力,抑制细胞迁移.

AIM：To investigate the influence of treatment with Sitengfang on cell adhesion,invasion and migration in human gastric carcinoma cell line SGC-7901.METHODS：SGC-7901 cells were cultured in vitro and treated with different concentration of Sitengfang（0,50,100 and 200 mg/L,respective-ly）.Cell adhesion was detected by CytoSelect TM 48-Well Cell Adhesion Assay.Cell invasion was,analyzed by Transwell invasion assay.Cell mi-gration was detected by scratch wound healing assay.RESULTS：Treatment with Sitengfang at a con-centration of 50,100 or 200 mg/L significantly inhibited the adhesion and invasion of SGC-7901 cells（adhesion：0.34±0.05,0.13±0.00,0.12±0.01 vs 1.34±0.12;invasion：39±3,26±6,8±2 vs 80 ±5,all P0.01）.Wound healing assay showed a significant reduction in the migration capacity of cells treated with 50 mg/L Sitengfang for 48 h（28.57%±4.10%vs 59.68%±5.98%,P0.01）.CONCLUSION：Treatment with Sitengfang sig-nificantly decreases cell adhesion,invasion and migration in human gastric carcinoma cell line SGC-7901.