中药提取物水苏糖对冰水灌胃应激后肠功能紊乱大鼠作用的实验研究

Experimental study of the stachyose, an extract of Chinese herbal medicine, in rats functional bowel disorder induced by stomach irritation with cool water

目的 观察中药提取物水苏糖对冰水灌胃应激后肠功能紊乱大鼠的排便粒数、粪便含水量、小肠推进率和肠道菌群的影响,探讨中药提取物水苏糖对应激后肠功能紊乱的作用及机制.方法 应用冰水灌胃应激法建立便秘型肠易激综合征大鼠模型,灌胃给予水苏糖1.5 g/（kg·bw）,检测给药第7天及第14天大鼠12 h内的粪便粒数、粪便含水量、小肠推进率的变化及给药14 d肠道菌群的变化.结果 水苏糖治疗组大鼠第14天12 h内的粪便粒数、粪便含水量及小肠推进率明显增加,肠道菌群中双歧杆菌、乳酸杆菌等优势菌群的数量明显增加,与便秘型肠易激综合征模型对照组比较差异有统计学意义[（38.43±4.57）粒VS（32.21±3.43）粒,F=3.892,P＜0.05;（47.88±3.43）%VS（43.18±6.85）%,F=6.724,P＜0.01;（1.04±0.11）弧度VS（0.88±0.08）弧度,F=4.965,P＜0.05;（10.77±0.44）1g（CFU/g） VS（9.85±0.43）lg（CFU/g）,F=7.613,P＜0.01;（10.96±0.35）lg（CFU/g）VS（9.84±0.35）lg（CFU/g）,F=10.413,P＜0.01].结论 中药提取物水苏糖具有改善冰水应激后肠功能紊乱大鼠便秘及调节肠道菌群功能的作用,调节机体微生态平衡可能是中药提.取物水苏糖治疗应激后肠功能紊乱的机制之一.

Objective To investigate the regulatory effects of stachyose through oral administration on the facilitating feces excretion function and its relationship to the intestinal flora in stomach irritation with cool water-induced functional bowel disorder rats. Methodes Stomach irritation with cool water -induced constipation-predominant irritable bowel syndrome model rats were administrated by i.g. with stachyose at intestine were determined within 12 hours on the 7th and 14th day after stachyose administration, and intestine flora was measured on the 14th day after Stachyose administration. Results The feces granules and the moisture contests of feces and the efficiency of the small intestine were increased in stachyose treated C-IBS rats, and the number of intestinal flora was significantly higher than that in the control C-IBS rats [ （38.43±4.57） grain VS （32. 21 ± 3.43） grain, F = 3.892, P ＜ 0.05; （47. 88 ± 3.43）% VS （43. 18 ±6.85）%, F =6.724, P ＜0.01;（1.04±0. 11）radian VS （0. 88 ±0.08）radian, F =4.965, P ＜0.05;（10.77 ±0.44）1g（CFU/g） VS （9.85 ±0.43）1g（CFU/g）, F =7.613, P ＜0.01;（10.96±0.35）lg（CFU/g） VS （9. 84 ±0. 35）1g（CFU/g）, F = 10. 413, P ＜0. 01 ]. Conclusion The results indicated that the stachyose,an extract of Chinese herbal medicine, had the facilitating feces excretion function and modulation functionon intestinal flora in stomach irritation with cool water-induced functional bowel disorder rats.