EZH2与乳腺癌及三阴性乳腺癌相关性的研究

Relationship between EZH2 and breast cancer and triple-negative breast cancer

目的:分析EZH2表达与乳腺癌及三阴性乳腺癌(TNBC)的相关性。方法:利用免疫组化法对82例乳腺癌和20例乳腺良性病变检测EZH2表达情况,收集82例乳腺癌的临床病理资料及TNBC表达情况,并与EZH2表达情况进行比较,Kaplan-Meier法分析乳腺癌的5年无瘤生存率。结果:乳腺良性病变中EZH2阳性表达率为20.0%,乳腺癌病例中EZH2阳性表达率为51.2%,两者比较差异有统计学意义,χ2=6.329,P=0.012;82例乳腺癌患者中,EZH2阳性表达患者与阴性表达患者比较,在肿瘤直径、组织学分级、淋巴结转移方面差异均有统计学意义(P<0.05),而在患者年龄、是否绝经、有无乳腺癌家族史方面差异无统计学意义,P>0.05。82例乳腺癌患者中,TNBC的EZH2阳性率为89.5%,非TNBC的EZH2阳性率为39.7%,两者差异有统计学意义,χ2=14.484,P=0.000;82例乳腺癌患者中,5年无瘤生存率EZH2阳性组为64.3%,EZH2阴性组为85.0%,差异有统计学意义,Log-rank=3.995,P=0.046。结论:EZH2阳性表达在乳腺癌患者中要高于乳腺良性病变,其在乳腺癌患者中比阴性表达患者更易复发转移,且与TNBC的发生更为密切。

OBJECTIVE：To investigate the expression of EZH2 in breast cancer and triple-negative breast cancer（TNBC） and their correlation.METHODS：A total of 20 cases of breast benign lesion and 82 cases of breast cancer were detected for the expression of EZH2 by the immunohistochemistry method,and their relationships were analyzed with the clinical pathologic characteristics and the expression of TNBC.The 5-year disease-free survival（DFS） was analyzed by Kaplan-Meier methods.RESULTS：The EZH2 expression was detected in 20.0% of breast benign lesion cases and in 51.2% of breast cancer cases,and the difference showed a statistically significance（χ2=6.329,P=0.012）.In the 82 cases of breast cancer patients,the expression of EZH2 was correlated with the tumor size,pathological grade and lymph node metastasis（P〈0.05）.In contrast,it did not show correlation with patients age,whether or not menopause and family history of breast cancer（P〈0.05）.The EZH2 expression was detected in 89.5% of TNBC and 39.7% of non-TNBC,and the difference showed statistical significance（χ^2=14.484,P=0.000）.The 5-year DFS was significantly lower in EZH2 positive patients than in EZH2 negative patients（64.3% vs 85.0%）,Log-rank=3.995,P=0.046）.CONCLUSIONS：The expression of EZH2 is higher in breast cancer than in breast benign lesion.In breast cancer patients,the patients with over expression of EZH2 have an increased possibility of recurrence or metastasis,and more intimate relationship with TNBC.