光照强度和氮素配比对葡萄试管苗氮素代谢的影响

Effect of Light Intensity and Nitrogen Ratios on Metabolism of Nitrogen of Grape Plantlets in vitro

以红地球葡萄试管苗为试材,探讨了光照强度和NH_4^+-N、NO_3^--N不同配比对葡萄试管苗氮代谢的影响。结果表明:随培养基中NH_4^+-N所占比例和光照强度的升高,GS活力增加。光强为5 000 lx并且NH_4^+-N:N_3^--N为5:5时,GS活力最高。硝酸还原酶活性(NR)随着光强和培养基中NO_3^--N比例的增加而升高,且随着培养基中NO_3^--N比例的增加,试管苗体内的NO_3^--N含量增加。随NR活性升高,体内NO_3^--N含量降低,NO_3^--N含量与NR活性之间呈显著的负相关关系。

The effect of light intensity and different nitrogen form and ratios on metabolism of nitrogen were studied.The grape plant lets in vitro Red Globe was cultivated with the media under three kinds of light intensities and three kinds of nitrogen.The results showed that GS（glutamine synthetase） activity increased with the increase of light intensity and the ratio of NH_4~＋-N.In comparison with other treatments GS activity was the highest,when the ratio of NH_4~＋-N to NO_3^--N was 5：5 under 5 000 lx light intensity.NRCNitrate Reductase） activity and NO_3^--N accumulation in grape plantlets in vitro decreased with the increase of the ratio of NO_3^--N.The activity of NR was increased by increased light intensity,at the same time nitrate accumulation decreased.Nitrate accumulation was significantly negatively correlated to the activity of NR.