铜胁迫对蚕豆幼苗基因组DNA损伤效应的研究

Study on DNA Damages in Vicia faba Seedings Under Copper Stress

采用AFLP分子标记技术,研究了Cu胁迫对蚕豆幼苗根系生长的影响和基因组DNA的损伤效应。结果表明:不同浓度(12.5、25、50 mg/L)Cu处理7 d后,蚕豆幼苗根长和根系内的可溶性蛋白含量均受到了抑制;基因组DNA的AFLP图谱发生显著变化,多态性增加,且与Cu浓度存在明显的剂量关系;Cu影响蚕豆幼苗根尖细胞基因组DNA的稳定性,利用AFLP技术获得的DNA多态性变化可作为土壤Cu污染的早期诊断。

Vicia faba seedlings growth and genomic DNA damage effects of copper stress were studied by amplified fragment length polymorphic（AFLP） technique.The results showed that after 7 days of 12.5,25,50 mg/L copper stress ,the root elongation growth was inhibited,and the total soluble protein content in root tip was decreased,compared to that of the normal seedlings（P0.05）.The AFLP profiles showed obvious differences between normal and exposed plantlets,included loss of normal bands and appearance of new bands and variation in band intensity.And the change effect was dose-dependent,and the DNA polymorphisms detected by AFLP technique could be used as a biomarker for detecting the genotoxic effects of copper pollution on organisms in soil system.