芪棱汤对大鼠脑缺血再灌注后一氧化氮的影响

Influence of Qileng Decoction on expression of nitric oxide after focal Cerebral Ischemic Reperfusion in Rats

目的:观察芪棱汤对脑缺血再灌注损伤后一氧化氮的影响,为益气养阴活血法治疗缺血性中风提供实验依据。方法:将大鼠随机分为假手术组、模型组、补阳还五汤组及芪棱汤组。采用颈内动脉线栓加环扎法复制大鼠脑缺血再灌注模型。于再灌注后不同时间点观察大鼠缺血脑组织的一氧化氮(NO)含量。结果:模型组NO含量较假手术组高(均P<0.01),补阳还五汤组、芪棱汤组的NO含量较模型组低(P<0.05),芪棱汤组在降低NO方面优于补阳还五汤组(P<0.05)。结论:芪棱汤可降低NO的含量,从而对脑缺血再灌注所导致的脑损伤起保护作用。

Objective To explore the Influence of Qileng Decoction on expression of nitric oxide after focal cerebral ischemic reperfusion in Rats,and to provide the scientific foundation for benefit vital energy（BVE）,nourishing in（NY）,activating blood circulation（ABC）on treating ischemic stroke.Methods The rats were randomly divided into four groups including sham-operation,model group,BuYangHuanWu（BYHW） decotion group and Qileng decoction group.The model of focal cerebral ischemic repergusion injury was made by using thread embolizing and fastening of the ICA in rats.the content of nitric oxide was measured by nitrate reductase method at different time point after cerebral ischemic reperfusion.Results the content of nitric oxide in model group was significantly higher than sham-operation group（P0.01）,and those in BuYangHuanWu decoction group and QiLeng decoction group decreased significantly compared with the model group（P0.05）.Contrasted with BuYangHuanWu decoction group,the content of NO in QiLeng decoction group decreased remarkably.Conclusion QiLeng decoction plays the nerve protective effect by reducing the content of nitric oxide,It may be one of the mechanism of BE-NY-ABC treating ischemic stroke.