2D-DIGE技术研究自交不亲和杏品种‘新世纪’花柱表达蛋白

Reaserch of Protein Expression of Style In Self-Incompatibility Cultivar Prunus armeniaca L.cv.Xinshiji by 2D-DIGE Technique

【目的】从蛋白质组水平探索杏树自交不亲和的分子遗传机制。【方法】采用双向差异凝胶电泳和质谱检测技术,对自交不亲和杏品种‘新世纪’在小蕾期、大蕾期、自花授粉后24 h及异花授粉(‘新世纪’ב巴旦水杏’)后24 h的花柱进行差异性蛋白质组学研究。【结果】在‘新世纪’杏的小蕾期、大蕾期、自花授粉后24 h、异花授粉24 h后的花柱中共检测到约1 500个蛋白点,其中有66个差异表达的蛋白点。自交不亲和杏柱头对自体与异体花粉感应在蛋白质组水平有差异,其中异花授粉后花柱中特异表达的蛋白点1个,异花授粉后表达丰度明显升高的蛋白点4个,表达丰度明显降低的蛋白点29个;质谱分析只有13个蛋白质点得到归属鉴定。【结论】明确‘新世纪’杏花花柱对自体与异体花粉感应在蛋白质组水平上有差异。

【Objective】The objective of the experiment is to explore self-incompatibility（SI） molecular mechanism of apricot at proteome level.【Method】Different proteomics of style at the stage of the small bud,big bud,24 h after self-pollination（ASP） and 24 h after cross-pollination（ACP）（＇Xinshiji＇×＇Badanshui＇） in the SI apricot ＇Xinshiji＇ by two dimension difference gel electrophoresis（2D-DIGE） and mass spectrum（MS）.【Result】About 1500 protein points were detected,and 66 different proteins were expressed at four different development stage of style in ＇Xinshiji＇.Self and non-self pollen had triggered different effects on style at proteomic level.There was one protein expressed special in cross-pollinated style,and four up-expressed and twenty-nine down-expressed in cross-pollinated style,respectively,compared with self-pollinated style.Thirteen protein spots were identified unambiguously.【Conclusion】 Different reactions on style at proteomic level had been obviously triggered in ＇Xinshiji＇ by self and non-self pollen.