摘要
目的:究动脉粥样硬化对SMC凋亡及凋亡相关基因表达的影响,探讨凋亡的分子机制及晚期AS斑块破裂之原因。方法:采用高维生素D3+高脂肪高胆固醇饲料喂养法建立大鼠AS的模型;运用DNA琼脂糖凝胶电泳法鉴定基因组DNA的断裂情况;使用Northern Blot检测P53、c-myc及bcl-2基因的表达活性。结果:a.AS斑块中SMC的DNA片断在电泳中呈特征性梯状分布;b.AS斑块中P53及C-myc基因转录明显增强;c.AS斑块中SMC的bcl-2mRNA含量显著下降。提示1.AS斑块中存在凋亡的SMC,P53及C-myc表达的增强和bcl-2的表达下调介导了这一过程;2.SMC的凋亡可能是晚期AS斑块玻裂的重要原因。
In the present study,atherosclerosis rat model was produced by feeding rats with high-VitD3 and cholesterol diet,identification of apoptosis in As plague was performed by DNA agarose gel clcctrophoresis analysis and levels of bcl-2,p53 and c-myc messenger RN'A were detected by Northern blot hybridization in order to elucidate the roles of these genesexpression changes in apoptotic death of SMCs in As plague and further explore the possible molecular mechanism involved in their apoptosis simultaneously with considerably enhanced expressions of p53 and c-myc genes and greatly decreased expression of bcl-2 gene.It's concluded that just the stable changes in endogenous expressions of proapoptotic genes p53 and c-myc as well as anti apoptotic gene bcl-2 initiate death programm of SNCs in As plague and SmCs apoptosis in the advanced As plague mayp romote plague rapture and the Subsequent thrombosis of vessel.
