两种方法定位5个地点大豆蛋白质含量QTL

QTL analysis of soybean protein content using two methods in 5 different environments

利用CIM和MIM法,对大豆Charleston×东农594的154个重组自交系在5个不同地点进行蛋白质含量测定和QTL定位分析。共检测到25个QTL,分别位于A1、B1、C2、D1a、D1b、E、J、L和N连锁群上。用CIM法定位了20个与蛋白质含量相关的QTL,用MIM法定位了9个与蛋白质含量相关的QTL。在C2连锁群上多次定位的ProC2-4,标记区间为Sat_092-Satt460,与前人找到的QTL位点一致。

Two mapping methods,composite interval mapping（CIM） and multiple interval mapping（MIM）,were employed to detect quantitative trait loci（QTL） of soybean protein content using 154 recombination inbred lines（RIL） population derived from a cross between Charleston and Dongnong 594 in five different locations.As a result,25 QTLs for protein content were mapped in linkage group A1,B1,C2,D1a,D1b,E,J,L,and N.In total,20 QTLs for protein content were detected by CIM,and 9 QTLs detected by MIM.Among the QTLs,ProC2-4 was mapped multiple times between Sat_092 and Satt460 on C2 linkage group,referred as a main effect QTL for soybean protein content.