金丝桃苷对皮质酮损伤的PC12细胞的保护作用

The Protective Effect of Hyperoside on the Cultured PC12 Cell Injury Induced by Corticosterone

目的利用PC12细胞的抗抑郁损伤模型研究金丝桃苷的抗抑郁作用及其可能机制。方法金丝桃苷、盐酸氟西汀与0.01 mmol.L-1皮质酮共孵PC12细胞48 h,采用形态学法、MTT比色法、LDH法、Fura-2/AM荧光标记法测定胞内Ca2+浓度。结果高浓度皮质酮处理PC12细胞后,细胞数显著增多,受损变圆的细胞数较少;OD490值显著升高(P<0.01),LDH释放量较少(P<0.01),胞内[Ca2+]i升高,表明细胞受到损伤或部分死亡;当给予金丝桃苷或盐酸氟西汀后,细胞存活率升高,表明细胞损伤减少或接近正常。结论与经典抗抑郁剂盐酸氟西汀的作用效果一样,金丝桃苷对皮质酮损伤的PC12细胞有明显的保护作用和抗抑郁作用,其机理可能与神经细胞的保护作用有关。

Objective To study the antidepressant effect of Hyperoside and its possible mechanism,the model of PC12 cell injury induced by high concentration corticosterone was carried out.Methods Cultured PC12 cells were treated with 0.01 mmol·L-1 corticosterone（Cort） for 48h at the presence of Hyperoside or Fluoxetine,morphological observation,the cellular viability,the release amount of LDH and intracellular free Ca2＋ concentration（i） were determined by MTT assay,LDH assay and Fura-2/AM fluorescence labelling respectively.Results After incubation with Cort 0.01mmol·L-1 for 48 h,Hyperoside attenuated round shaped cells induced by corticosterone,OD490 was increased significantly,LDH release from PC12 was decreased,the was overloaded.Conclusion Treatment with Hyperoside can protect PC12 cell injury induced by Cort just the same as classical antidepressant Fluoxetine.