牛珀至宝丹对老年大鼠脑内bFGF阳性神经元的影响

Influence of NPZBD on bFGF Positive Neurons in Aged Hypomnesia Rat Brain

目的探讨牛珀至宝丹对老年大鼠学习记忆能力及脑尾壳核和皮质区bFGF阳性神经元的影响。方法以成年大鼠平均逃避潜伏期的均值加两倍标准差和加一倍标准差为参照标准,将老年大鼠随机分为记忆正常组、记忆减退组、牛珀小剂量组及牛珀大剂量组。牛珀至宝丹0.05,0.1 g/（kg.d）连续给药15 d后,Morris水迷宫进行学习记忆能力检测,免疫组化检测尾壳核及皮质区bFGF阳性神经元数量的变化。结果定位航行实验第4~5天,牛珀大剂量组大鼠平均潜伏期明显缩短,与记忆减退组比较,有显著性差异（P〈0.05）;空间探索实验,牛珀大剂量组大鼠第Ⅱ象限停留时间、第一次平台穿越时间、穿越平台次数与记忆减退组比较,有显著性差异（P〈0.05）;牛珀大剂量组大鼠脑尾壳核和皮质区bF-GF阳性神经元明显增多,与记忆减退组比较,有极显著性差异（P〈0.01）。结论牛珀至宝丹对老年记忆减退大鼠学习记忆能力有明显促进作用,其机理可能与牛珀至宝丹增加脑尾壳核及皮质区bFGF阳性神经元的数量有关。

Objective To discuss the influence of NPZBD on ability of learning and memory and bFGF positive neurons in Caudate putamen and cortex of aged hypomnesia rats.Methods The aged rats were randomly divided into normal memory group,hypomnesia group,NPZBD low dose treatment group and NPZBD large dose treatment group.The aged rats of treatment group were administered with NPZBD 0.05 g/（kg·d） and 0.1 g/（kg·d）for 15 d.All rats were tested ability of learning and memory with Morris water maze after the administration and detected the changes of bFGF Positive neurons in Caudate putamen and cortex with immunohistochemistry.Results In 4th to 5th day of splace navigation,the escape latency of rats in NPZBD large dose treatment group was significantly shorter than that of hypomnesia group,there was significant difference（P0.05）;in space probe test,the time in second quadrant,time of first through platform,times of through platform of rats in NPZBD large dose treatment group was significantly different with the rats in hypomnesia group（P0.05）.The bFGF positive neurons in Caudate putamen and cortex of aged hypomnesia rats in NPZBD large dose treatment group was significantly increased than those of hypomnesia group（P0.05）.Conclusion NPZBD has obvious promotion effect on ability of learning and memory in aged hypomnesia rats.The mechanism might be connected with increased bFGF positive neurons in Caudate putamen and cortex of aged hypomnesia rats.