泽兰大孔树脂洗脱部位中原儿茶醛、咖啡酸和迷迭香酸含量的反相高效液相色谱法测定

HPLC Determination of Protocatechualdehyde,Caffeic Acid,Rosmarinic Acid in the Eluate of Herba Lycopi by Macroporous Resins

目的建立同时测定泽兰中原儿茶醛、咖啡酸、迷迭香酸的高效液相色谱含量测定方法,并对泽兰大孔树脂洗脱部位中的这3种酚酸的含量进行了测定。方法采用Cadenza（5）CD-C18（250 mm×4.6 mm×5μm）分析柱;流动相为乙腈/水系统,两相均含有0.75%的甲酸,以有机相10%起始,保持5 min后,于17 min内线性变为13%,于27 min内线性变为18%,于47 min内线性变为23%,于65 min内线性变为55%;流速1.0 ml/min;柱温30℃;于325 nm,280 nm双波长下检测。结果原儿茶醛（280 nm）、咖啡酸（325 nm）和迷迭香酸（325 nm）分别在0.01~2μg、0.01~2μg和0.04~8μg内呈良好的线性关系。3个成分相应的富集部位含量分别达到原儿茶醛0.141%,咖啡酸1.092%,迷迭香酸11.101%。结论该方法灵敏快速,准确可靠,可用于对泽兰及其大孔树脂洗脱工艺过程进行质量控制。

Objective To develop a new method for determination of protocatechualdehyde,caffeic acid,rosmarinic acid in Herba Lycopi by HPLC and analyze the samples from the eluate of Herba Lycopi.by macroporous resins.Methods Eluate was carried on a Cadenza（5）CD-C18（250 mm×4.6 mm×5 μm）column with CH2CN-H2O（Two phase both contained 0.75% formic acid） as mobile phase by a gradient elution at double wavelength 325 nm and 280 nm.Results and Conclusion The method is sensitive and accurate with good repeatability and suitable for the quality control of eluate of Herba Lycopi Turcz..