红树林海洋淤泥中放线菌的分离与鉴定

Identification and Analysis of Actinomycetes in Marine Mud of Mangrove

为了解广西北海红树林海洋淤泥中的放线菌资源,采用海水配制高氏培养基分离红树林海洋淤泥中的放线菌样品,从中筛选、分离、提取10株典型放线菌菌株总DNA,用放线菌通用引物对16Sr DNA进行PCR扩增,对获得的扩增结果进行DNA序列测定和对比鉴定。结果表明,10株典型放线菌菌株为2种菌属,其中有8株为链霉菌属(80%),为常见放线菌;有2株为拟诺卡氏菌属(20%),为稀有放线菌。表明,广西北海红树林海洋淤泥蕴含着种类丰富的放线菌。

To explore actinomycetes resources in marine mud of mangrove in Beihai city of Guangxi province,actinomycete samples in marine mud of mangrove were separated by using seawater prepared Gause culture medium,total DNA of 10 typical strains of actinomycetes was screened,separated and extracted,16Sr DNA was amplified by PCR with universal primers.The results from DNA sequencing and comparison identification of amplified product show that 10 typical strains of actinomycetes belong to 2 genera,8 of which are common actinomycetes and belong to Streptomyces（80%）,the other 2 are rare actinomycetes and belong to Nocardia（20%）.All above indicate that there are abundant species of actinomycetes in marine mud of mangrove in Beihai city of Guangxi province.