肝蛋白提取纯化及应用研究进展

Review on Extraction,Purification and Application of Liver Protein

介绍了肝蛋白的主要提取方法,即水溶液提取法、碱提酸沉法、有机溶剂提取法、蔗糖密度梯度离心法、超声波萃取法等;分离纯化方法,即盐析法、低温有机溶剂沉淀法、透析与超滤、凝胶过滤法、亲和层析法、电泳法、离子交换层析法等。同时阐述了肝蛋白的应用及其应用前景。

The main extracting methods of liver protein which including aqueous extraction,acidic distillation,organic solvent extraction,sucrose density gradient centrifugation,ultrasonic extraction and so on,and the separation and purification method which including slating out,organic solvent precipitation,dislysis and ultra-filtration,gel filtration,affinity chromatography,electrophoresis,ion exchange and so on are introduced in this paper,and the application and prospects of liver protein are reviewed as well.