脊髓和周围神经系统中内源性大麻酚类花生四烯甘油的液相层析-质谱检测(英文)

Liquid chromatographic-mass spectrometric measurement of the endogenous cannabinoid 2-arachidonylglycerol in the spinal cord and peripheral nervous system

AIM: To develop a sensitive method for measuring theputative endocannabinoid 2-arachidonylglycerol (2-AG)in the peripheral and ceatral nervous system.METHODS: A method using atmospheric pressurechemical ionization (APCI) liquid chromatography/mass spectrometry (LC/MS ) was developed todetermine the levels of 2-AG in methanol extracts of therat lumar spinal cord, dorsal root ganglion (DRG),and sciatic nerve. RESULTS: 2-AG was detectedwith high sensitivity and minimal sample preparation.The levels in the tissues analyzed were ≤pmol/mg wetweight. Similar levels were found in the spinal

AIM: To develop a sensitive method for measuring the putative endocannabinoid 2-arachidonylglycerol (2-AG) in the peripheral and central nervous system. METHODS: A method using atmospheric pressure chemical ionization (APCI) liquid chromatography/ mass spectrometry ( LC/MS ) was developed to determine the levels of 2-AG in methanol extracts of the rat lumbar spinal cord, dorsal root ganglion (DRG), and sciatic nerve. RESULTS: 2-AG was detected with high sensitivity and minimal sample preparation. The levels in the tissues analyzed were ≤pmol/mg wet weight. Similar levels were found in the spinal cord and the DRG, whereas approximately 7-fold lower levels were observed in the sciatic nerve. CONCLUSION: 2-AG is present in the peripheral nervoussystem, and the levels are markedly higher in cell bodies than those in axons.