白腐菌Trametes Hirsuta产漆酶用于含氯酚类废水的研究

Treatment of Wastewater Containing Chlorophenols with Laccase from White-rot Fungus Trametes Hirsuta

制浆造纸工业中氯漂废水中含有大量氯化木素等氯代酚化合物,这类有毒物质相当一部分被排放到水体中对环境造成严重威胁。本文以24,-二氯苯酚为氯酚类化合物的模型物,研究了处理时间,反应体系的温度、pH值对白腐菌所产漆酶对24,-二氯苯酚去除效果的影响,结果表明反应的最适温度为35℃,最适pH为4.5。在最佳条件下曝气20h,粗漆酶液对24,-二氯苯酚的去除效果可达到93.6%,此外,还对粗漆酶液进行了分离纯化,比较了纯化后漆酶与粗酶液对24,-二氯苯酚的去除效果,结果表明粗酶液的处理效果比纯化后的更好。

Lignin chloride and related chorophenols are main toxic organic pollutants in the effluent of chlorine bleaching in paper industry. A part of the wastewater containing chlorophenols is discharged into environment and causes pollution. In this work, 2,4 -dichlorophenol （2,4 -DCP） was used as typical chlorinated aromatic com- pounds in the wastewater, the removal of 2,4 - DCP was investigated with catalyzer of laccase from a white - rot fungus, i.e. Trametes hirsuta. The results show that the treatment time, reaction temperature and pH are important impact factors on the removel rate of 2,4 -DCP. The optimal conditions are as follows： 35 ~C, pH 4.5. With the optimal condition, after aeration for 20h, the removal rate of 2,4 - DCP catalysed by crude laccase can reach 93. 6%. Moreover, the crude enzyme was separated and purified. However, the purified laccase had lower removal ef- ficiency than the crude enzyme at the same reaction condition.