悬铃木ISSR反应体系的建立

ESTABLISHMENT OF ISSR REACTION SYSTEM OF PLATANUS ACERIFOLIA

为了探讨适合悬铃木的ISSR体系,选育无球少球悬铃木,本实验以悬铃木幼嫩叶片为实验材料,通过对CTAB方法的改良,摸索出适于悬铃木基因组DNA提取的方法,并以核酸纯度、片断分布情况等指标来评价,获得高质量基因组DNA;用2个不同样品对16对ISSR引物进行了筛选,并应用其中的4对ISSR引物对15株悬铃木进行了初步应用,获得了清晰稳定的扩增图谱。上述结果表明获得的基因组质量较高,同时也表明反映体系对悬铃木的ISSR分析是可行的;并对影响悬铃木基因组DNA的制备及扩增反应因素进行了简要的分析讨论。

To probe the effective ISSR system and to breed none - or - little cones Platanus acerifolia, genomic DNA was extracted with modified CTAB method from tender leaves of Platanus acerifolia. DNA quality was e- valuated by indexes like purity and distribution of nucleic acid. High - quality genomic DNA was obtained. Sixteen ISSR primers were screened with 2 different samples. 4 ISSR primers were adopted to evaluate preliminaryinitially among 15 plane trees and clear and stable amplification patterns was obtained,which indicated the DNA was good and the system was feasible. The factors affecting the DNA extraction and amplification of genome DNA of Platanus acerifolia were discussed in the paper.