黑龙江地区野生黑木耳菌种的ISSR指纹分析

ISSR fingerprint analysis of wild strains of Auricularia auricula in Heilongjiang Province

为研究黑龙江省野生黑木耳之间的亲缘关系采用ISSR标记对来自黑龙江省各地区的24个野生木耳菌种和一个栽培菌种进行DNA指纹图谱分析,在ISSR的基础上用NTSYS软件进行聚类分析,相似水平在0.49时,可将25个供试黑木耳菌株分为4个组群。菌种亲缘关系按照区域分布明显,实验结果表明,黑龙江省野生黑木耳菌株遗传背景在不同的区域内有一定差异,在相同区域内遗传背景相似度接近,但个别产地相邻的菌种亲缘关系也差异明显,栽培菌种与野生菌种的相似性很低。研究表明,ISSR可以有效地用于黑木耳野生菌株的快速准确鉴别,是用来研究黑木耳指纹图谱分析的理想方式。同时也为分析野生黑木耳遗传背景提供一定的基础依据。

In order to study the genetic relationship between the wild Auricularia auriculars in Heilongjiang Province,the paper used ISSR molecular markers to analyze 24 wild Auricularia auriculars of various regions of Heilongjiang Province and DNA finger-print of one cultivation Auricularia auriculars strain. To use NTSYS software conduct the cluster analysis on the basis of ISSR and divided 25 tested Auricularia auriculars strains into four groups under the similarity level of 0.49. Experimental result showed that the genetic relationship of wild Auricularia auriculars strains in Heilongjiang Province distribution apparently according to regional difference,genetic backgrounds in different areas had a certain difference and had a close similarity within the same region. But a few fungus strains which had adjacent producing area showed significant genetic relationship differences and the similarity between cultivated strains and wild strains had a large difference. Studies showed that,ISSR could be effectively used for identify the wild Auricularia auriculars strains fatly and accurately. It was not only an ideal way to study the Auricularia auriculars＇ finger-print but also provided the basis for the analysis of the wild Auricularia auriculars.