肝硬化患者血清谷氨酰胺合成酶活性检测

Activity detection of glutamine synthetase in liver cirrhosis patients

目的探讨肝硬化患者血清谷氨酰胺合成酶(GS)的活性变化及其临床意义。方法采用终点法测定48例肝硬化患者血清GS的活性;计算GS诊断肝硬化的阳性率、阴性率、灵敏度和特异性;并分析血清GS与清蛋白(ALB)、丙氨酸氨基转移酶(ALT)、总胆红素(TB)的相关性。结果肝硬化组血清GS活性为(13.28±3.62)U/mgprot,正常组血清GS活性为(6.62±3.23)U/mgprot,二者差异有统计学意义(P<0.001),但GS活性与ALB、ALT、TB均无相关性。结论肝硬化患者GS活性增高,可作为肝硬化诊断和鉴别诊断的参考指标。

Objective To evaluate the activity and clinical significance of glutamine synthetase（GS）in liver cirrhosis patients.Methods The activity of GS in 48 liver cirrhosis patients was detected by endpoint method.The positive rate,negative rate,sensitivity and specificity of GS in the diagnosis of liver cirrhosis were calculated.To compare the correlation between GS with ALB,ALT and TB respectively.Results The activity of GS was（13.28±3.62）U/mgprot in cirrhosis group and（6.62±3.23）U/mgprot in normal group with significant difference between the two groups（P0.001）.GS had no correlation with ALB,ALT and TB.Conclusion The activity of GS in liver cirrhosis patients is increased.GS can serve as reference index in diagnosis and differential diagnosis of liver cirrhosis.