SDS-聚丙烯酰胺凝胶电泳鉴定降纤酶纯度的研究

Purity Assay for Defibrase by Polyacrylamide Gel Electrophoresis

采用快速凝胶电泳方法同时完成蝮蛇抗栓酶、精制蝮蛇抗栓酶和降纤酶定性的鉴定，同时采用高效液相色谱方法进一步鉴定其纯度的相对百分含量为90％以上，色谱峰为一对称性单峰，分子量36000，为降纤酶生产过程中控制产品质量提供一种简便、快速特异性的分析方法。

A method of gel electrophoresis has been applied to the qualitative assay of Phospholipase A2, purified Phospholipase A2 of Agkistrodon halys and Defibrase at the same time. Meanwhile a HPLC method was used to prove that the purity of the three products are above 90%. A couple of symmetric single Peaks at molecular weight of 36000 were observed in this HPLC test. This method contributes a reliable analysis for QC in Defibrase production and it is simple, specific and prompt.