肝病患者外周血血管生成素-2定量检测的临床价值分析

Expression of circulating angiopoietin-2 and its clinical values in sera of patients with liver diseases

目的观察肝病患者外周血中血管生成素-2(Ang-2)的表达水平,探讨与肝癌(HCC)发展关系及临床价值。方法收集不同肝病患者外周血标本,以酶联免疫吸附法(ELISA)检测Ang-2浓度,并分析与血管内皮生长因子(VEGF)相互关系。结果肝病患者血Ang-2水平,正常对照组为(17.4±2.6)ng/ml、急性肝炎组为(23.5±6.5)ng/ml、慢性肝炎组为(20.9±7.1)ng/ml,肝硬化组为(25.5±5.8)ng/ml和肝癌组为(40.8±3.5)ng/ml;Ang-2水平随肝病进展呈进行性增加,肝癌组明显高于对照组和良性肝病组(P<0.001),且Ang-2与VEGF改变呈显著正相关(r=0.769,P=0.026);如以血Ang-2>35ng/ml为界,肝癌组95%异常,肝硬化组1例异常(2.9%),其他组未见异常,与AFP联检对肝癌具有互补诊断价值。结论 Ang-2过表达与肝癌发展密切相关,且有助于肝癌的诊断与鉴别。

Objective To investigate the correlation between expression of circulating angiopoietin-2 （Ang-2） and hepatocellular carcinoma （HCC） development and diagnostic values in patients with liver diseases. Methods The expression of circulating Ang-2 and VEGF was analyzed by ELISA and explored their relationship. Results The progressing increasing of circulating Ang-2 expression from normal subjects （ 17. 4 ± 2. 6） ng/ml to acute hepatitis （ 23. 5± 6. 5 ） ng/ml, chronic hepatitis （ 20. 9 ± 7. 1 ） ng/ml, liver cirrhosis （25.5 ±5.8） ng/ml and HCC （40. 8 ±3. 5） ng/ml. Significant differences was found between HCC patients and other liver diseases or control subjects （ P 〈 0. 001 ） and closely positive relationship between Ang-2 and VEGF （ r = 0. 769, P = 0. 026 ）. If cutoff value of serum Ang-2 expression was more than 35 ng/ml, higher incidence was found in 95% of HCC patients and lower expression in other liver diseases except of one case with cirrhosis. Combined serum Ang-2 and AFP analysis should be useful markers for HCC diagnosis. Conclusions Ang-2 overexpression is associated with HCC development and should be an useful biomarker for HCC diagnosis or differential diagnosis.