非酒精性脂肪性肝病兔肝组织甘油三酯、丙二醛、超氧化物歧化酶与血浆内皮素的关系

Relations of triglyceride,malondialdehyde and superoxide dismutase in the rabbit liver tissue,ET-1 in the plasma with nonalcoholic fatty liver disease

目的检测非酒精性脂肪性肝病(NAFLD)兔肝组织甘油三酯(TG)、丙二醛(MDA)、超氧化物歧化酶(SOD)浓度与血浆内皮素-1(ET-1)之间的关系,探讨TG、MDA、SOD与ET-1在NAFLD发病中的相互作用。方法 40只日本大耳白兔数字法随机分为重度NAFLD组(重度组)、轻度NAFLD组(轻度组)、空白对照组(对照组)。重度组给予高脂饲料160g·兔-1·d-1,轻度组给予高脂饲料80g·兔-1·d-1+普通饲料80g·兔-1·d-1,对照组给予普通饲料160g·兔-1·d-1。饲养13周。实验前后采集血浆标本,检测TG、胆固醇(TC)、ET-1;检测肝组织匀浆MDA、SOD、TG浓度;肝组织HE染色,光镜观察肝脏病理学。结果 (1)血浆TC、TG:饲养后重度组TC、TG分别为(32.12±1.25)mmol/L、(6.02±2.12)mmol/L,轻度组分别为(18.34±2.10)mmol/L、(4.39±1.93)mmol/L,与饲养前比较差异有统计学意义(P<0.01);重度组TG、TC高于轻度组(P<0.01)。(2)肝组织TG:重度组(0.71±0.07)mmol/L、轻度组(0.52±0.08)mmol/L,与对照组(0.29±0.10)mmol/L比较差异有统计学意义(P<0.01),重度组与轻度组比较差异有统计学意义(P<0.01)。(3)肝组织MDA浓度:重度组(219.87±25.57)nmol·mg-1·pro-1、轻度组(154.91±26.98)nmol·mg-1·pro-1,与对照组(99.95±20.87)nmol·mg-1·pro-1比较差异有统计学意义(P<0.01),重度组与轻度组比较差异有统计学意义(P<0.01)。(4)肝组织SOD活性:重度组(27.49±8.17)nU·mg-1·pro-1、轻度组(48.76±7.37)nU·mg-1·pro-1,与对照组(64.47±7.89)nU·mg-1·pro-1比较差异有统计学意义(P<0.01),重度组与轻度组比较差异有统计学意义(P<0.01)。(5)血浆ET-1浓度:重度组、轻度组ET-1明显上升,与对照组比较差异均有统计学意义(P<0.01),重度组与轻度组比较差异有统计学意义(P<0.05)。(6)肝脏病理学:重度组呈重度NAFLD,轻度组呈轻、中度NAFLD,对照组为正常肝脏组织。结论 TG沉积量、脂质过氧化及血浆ET-1参与NAFLD的发生、发展,降低TG在肝脏的沉积,抑制过氧化反应,降低血浆ET-1浓度对抑制NAFLD的发生、发展有重要作用。

Objective To observe the relationships of triglyceride, malondialdehyde, superoxide dismutase in the rabbit liver tissue, and ET-1 in the plasma with nonalcoholic fatty liver disease, discusses the effects of TG, MDA, and SOD in the occurrence of NAFLD. Methods 40 Japanese white rabbits were randomly divided into severe group with NAFLD （ severe group）, mild group with NAFLD （ mild group）, blank control group（blank group）. Severe group was given high fat diet 160 g -1. rabbit-1 · day-1 , mild group were given high fat diet 80 g· rabbit- 1 . day- 1and normal diet 80 g · rabbit- 1 · day- 1, blank group was given normal diet 160 g·rabbit-1·day-1 The raising cycle is 13 weeks. Plasma samples were collected before and after experiment, tested triglyceride （TG） and cholesterol（TC）. The density of MDA, SOD, TG in liver tissue were tested. HE staining, pathological changes of liver tissue were observed with the aid of light microscope. Results （ 1 ） TC and TG level： after the feeding severe group of TC and TG respectively is （32. 12 ± 1.25）mmol/L, （6. 02 ± 2. 12）mmol/L, mild group of TC and TG respectively is （ 18.34 ±2. 10） mmol/L, （4. 39± 1.93 ） mmol/L, After the feeding, there was a significant difference compared in every group （P〈0. 01）. Severe group of TG and TC higher than mild group, there was a significant difference compared （P 〈 0. 01 ）. （2） The level of TG in liver tissue ： Severe group of TG in liver tissue is （0.71 ± 0. 07 ）mmol/L, mild group of TG in liver tissue is （0. 52± 0. 08）mmolfL, there was a significant difference compared with blank control group （P 〈 0. 01 ）, blank group of TG in liver tissue is （0. 29 ±0. 10） mmol/L, there was a significant difference between severe group and mild group （ P 〈 0.01 ）. （3） The level of MDA in liver tissue ： Severe group of MDA in liver tissue is （219. 87 ± 25. 57 ） nmol· mg-1· pro -1, mild group of MDA in liver tissue is （154. 91 ± 26. 98） nmol·mg-1· pro-1 , blank group of MDA in liver tissue is （99.95 ±20. 87） nmol · mg-1· pro-1, there was a significant difference compared with blank group （P 〈0. 01 ）, there was a significant difference between severe group and mild group（ P 〈 0. 01 ）. （4）The level of SOD in liver tissue： Severe group of SOD in liver tissue is （27.49 ± 8. 17）nmol·mg-1· pro-1 , mild group of SOD in liver tissue is （48.76 ±7.37） nmol· mg-1· pro-1 , blank group of SOD in liver tissue is （64. 47 ± 7. 89 ） nmol· mg - 1·Pro - 1, there was a significant difference compared with blank group （P 〈 0. 01 ）, there was a significant difference between severe group and mild group （P 〈 0. 01 ）. （5）The level of ET-1 in the plasma： Severe group and mild group significantly decreased in the plasma, there was a significant difference compared with blank group（P 〈0. 01 ）, there was a significant difference between severe group and mild group （P 〈 0. 05 ）. （6） Liver pathology： severe group of moderate was severe NAFLD, mild group was mild to moderate NAFLD, blank group was normal liver. Conclusions Triglyceride deposition amount, lipid peroxidation and plasma ET-1 participated the occurrence and development of NAFLD, they are very important to reduce the amount of triglyceride in liver, inhibit the peroxidation, reduce the level of plasma ET-1 for restraining the occurrence and development of NAFLD.