黄芪对周围神经雪旺氏细胞表达的影响

The effect of Astragalus peripheral nerve to Schwann cells

目的:探讨黄芪、甲基强的松龙干预对于检测雪旺氏细胞表达的影响。方法:无菌条件下取SD大鼠的坐骨神经、臂丛神经培养雪旺氏细胞,分三组分别加入黄芪、甲基强的松龙干预,每孔液体总量为500ul;另外一组为单纯雪旺氏细胞,作为对照组。分别培养24,48,72小时后,采用MTT法测细胞数量并作比较分析。结果:黄芪组日细胞平均数量随时间延长不断增长;培养24小时后,黄芪组日细胞数量明显高于对照组,而甲基强的松龙组细胞数量与对照组比较无统计学意义;培养48小时和72小时后,黄芪组日细胞平均数量明显高于对照组,而甲基强的松龙组细胞数量明显低于对照组。结论:作为神经恢复的重要指标,黄芪干预后雪旺氏细胞数量增加、细胞活性提高,说明黄芪对于雪旺氏细胞的表达具有积极意义。

Objective： To dicuss the effect of astragalus and jiajiqiang de songlong for the detection of Schwann cells. Methods： SD rats were obtained under sterile conditions the sciatic nerve, brachial plexus Schwann cell culture, astragalus and jiajiqiang de songlong were added in three groups intervention, 500ul per well total liquid; The other group,chwann cells alone as the control group. Were cultured 24,48,72 hours, the cell number by MTT method and comparative analysis. Result： RA group at the average number of cells growing with time, for 24 hours, cells in RA group was significantly higher at, the number of MP cells compared with the control group no significant differences. Cultured for 48 hours and 72 hours later, the RA group was significantly higher on the average number of cells, the jiajiqiang de songlong group of cells was significantly lower than control group. Conclusion： As an important indicator of nerve recovery, astragalus intervention to increase the number of Schwann cells, cell activity increased, indicating that Astragalus for Schwann cells positive.