不同分化培养基对秋水仙碱处理大葱愈伤组织的影响

Effects of Different Differentiation Media on Welsh Onion Callus

采用正交设计,测定了不同分化培养基对秋水仙碱处理大葱愈伤组织的影响。结果表明,在不添加2,4-D的基础上,适当提高蔗糖浓度,可以减缓愈伤组织褐化现象的发生,提高芽分化率,以处理6(蔗糖40 g/L+琼脂15 g/L+6-BA 1.5 mg/L)加倍诱导率最高。试验得到了两种嵌合体植株,一种为同时具有三倍体和二倍体细胞的植株,另一种为同时具有四倍体和二倍体细胞的植株。

The effects of different differentiation media on welsh onion callus treated with colchicin were determined by the orthogonal design experiment.The results showed that the appropriate increase of sucrose without 2,4-D could slow down the occurrence of browning phenomenon and increase the bud differentiation rate.The highest doubling induction rate could be obtained on the medium with 40 g/L sucrose,15 g/L agar and 1.5 mg/L 6-BA.Two kinds of chimera plants were acquired,which were the one with both triploid and diploid cells and the other one with both tetraploid and diploid cells.