Pak1基因在乳腺癌组织中的表达及临床意义

Expression of Pak1 gene in breast carcinoma and its clinical significance

目的研究乳腺肿瘤组织中p21激活激酶1(Pak1)mRNA及其蛋白在正常乳腺组织、良性乳腺肿瘤组织、乳腺癌组织中表达的情况,探讨Pak1与淋巴结转移、雌激素受体(ER)、孕激素受体(PR)及癌胚表达抗原(CerbB-2)表达的关系。方法采用实时荧光定量RT-PCR技术检测35例乳腺癌和20例乳腺良性病变、15例远癌正常乳腺小叶组织中Pak1 mRNA的丰度表达,免疫组化法检测35例乳腺癌和20例乳腺良性病变组织标本中Pak1蛋白的过度表达阳性率,并进行相关统计分析。结果乳腺癌、乳腺良性病变和正常乳腺小叶组织中Pak1 mRNA表达量分别为4.76±0.11、2.66±0.10、0.94±0.06,各组间比较差异有统计学意义(P<0.05)。在ER阳性与ER阴性组织中,Pak1 mRNA相对表达量为5.04±0.69、4.59±0.57,二者比较差异有统计学意义(P<0.05)。35例乳腺癌组织标本中,Pak1蛋白在ER阳性与ER阴性组织中过度表达阳性率分别为90.48%(19/21)和57.14%(8/14),二者比较差异有统计学意义(P<0.05);在PR阳性与PR阴性组织中过度表达阳性率分别为86.96%(20/22)和53.85%(7/13),二者比较差异有统计学意义(P<0.05)。Pak1 mRNA相对表达量和蛋白的过度表达阳性率均与乳腺癌患者年龄、肿瘤大小、CerbB-2表达水平和淋巴结是否转移未见相关性(P>0.05)。结论 Pak1基因表达增强是乳腺癌的普遍现象,且可能与ER、PR水平有关。

Objective To investigate mRNA and protein expression of Pak1 gene in normal breast tissue,benign mammary lesions and breast carcinoma,to explore whether the expression of Pak1gene in breast carcinoma is related to lymph node metastases,the level of ER,PR and CerbB-2 or not.Methods The mRNA expression of Pak1 gene was detected in 35 cases of breast carcinoma,20 cases of benign mammary lesions and 15 cases of normal breast tissue by fluorescent real-time RT-PCR;35 cases of paraffin-embedded specimens of breast carcinoma and 20 cases of benign mammary lesions were studied by immunohistochemical assay.Statistical analysis was carried out to analyze the correlation between Pak1 gene expression and clinical significance in these breast cancer patients.Results The mRNA expressions of Pak1 gene were detected in 35 cases of breast carcinoma,20 cases of benign mammary lesions and 15 cases of normal breast tissue by fluorescent real-time RT-PCR relative expression level of Pak1 mRNA were 4.76±0.11,2.66±0.10,0.94±0.06（P0.05）;the relative expression level of Pak1 mRNA in breast carcinoma with ER positive and ER negative were 5.04±0.69,4.59±0.57（P0.05）;the protein expression of Pak1 was detected in 35 cases of breast careinoma and 20 cases of benign mammary lesions by immunohistochemical assay.The over expression rate of Pak1 in breast carcinoma with ER positive and ER negative were 90.48%（19/21） and 57.14%（8/14,P0.05）;in breast carcinoma with PR positive and PR negative were 86.96%（20/22） and 53.85%（7/13,P0.05）;neither mRNA nor protein expression of Pak1 gene was correlated with age of patients,size of tumor,the level of CerbB-2（P0.05）;Conclusion Pak1 over expression is a common phenomenon in breast cancer,and may be related to the level of ER and PR of breast carcinoma.