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细胞抽提物重编程作用的研究进展 被引量：2

Epigenetic Reprogramming of Nuclei Using Cell Extract
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摘要 细胞抽提物重编程是近年来发展起来的技术,是将供体细胞抽提物导入受体细胞中,使受体细胞向供体细胞的形态、基因、功能等方面发生改变。目前,此方法已成功应用于体细胞与体细胞之间、体细胞与干细胞之间,成为一种有效的、无伦理学争议的重编程方法。本文就细胞抽提物重编程的研究现状、相关机理及存在的问题进行综述。 Epigenetic reprogramming of nuclei using cell extract becomes popular in recent years.This method indicates that recipient cells receiving transduction of cell extract from donor cells can generate changes of morphology,gene expression and function directing donor cells.It is reported to be used between somatic cells and somatic cells or stem cells as an effective method with no ethical controversy.The current situation,relevant mechanisms and defects concerning reprogramming of cell extract were reviewed.
作者 唐新杰 谢峰 张群
机构地区 上海交通大学医学院附属第九人民医院整复外科
出处 《组织工程与重建外科杂志》 2011年第1期48-50,共3页 Journal of Tissue Engineering and Reconstructive Surgery
基金 国家自然科学基金(30900308)
关键词 细胞抽提物 重编程 诱导 Cell extract Reprogramming Induction
分类号 Q785 [生物学—分子生物学]
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参考文献26

  • 1Wilmut I,Schnieke AE,McWhir J,et al.Viable offspring derived from fetal and adult mammalian cells[J].Nature,1997,385(6619):810-813.
  • 2Wakayama T,Perry AC,Zuccotti M,et al.Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei[J].Nature,1998,394(6691):369-374.
  • 3Gurdon JB,Byrne JA.The first half-century of nuclear transplantation[J].Proc Natl Acad Sci USA,2003,100(14):8048-8052.
  • 4Do JT,Han DW,Scholer HR.Reprogramming somatic gene activity by fusion with pluripotent cells[J].Stem Cell Rev,2006,2(4):257-264.
  • 5Tada M,Takahama Y,Abe K,et al.Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells[J].Curr Biol,2001,11(19):1553-1558.
  • 6Takahashi K,Yamanaka S.Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J].Cell,2006,126(4):663-676.
  • 7Aoi T,Yae K,Nakagawa M,et al.Generation of pluripotent stem cells from adult mouse liver and stomach cells[J].Science,2008,321(5881):699-702.
  • 8Park IH,Zhao R,West JA,et al.Reprogramming of human somatic cells to pluripotency with defined factors[J].Nature,2008,451(7175):141-146.
  • 9Rajasingh J,Lambers E,Hamada H,et al.Cell-free embryonic stem cell extract-mediated derivation of multi-potent stem cells from NIH3T3 fibroblasts for functional and anatomical ischemic tissue repair[J].Circ Res,2008,102(11):e107-117.
  • 10Walev I,Bhakdi SC,Hofmann F,et al.Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O[J].Proc Natl Acad Sci USA,2001,98(6):3185-3190.

二级参考文献6

  • 1徐荣辉，中华骨科杂志，1994年，14卷，185页
  • 2徐荣辉，中西医结合杂志，1991年，11卷，668页
  • 3柴本甫，中华实验外科杂志，1985年，2卷，157页
  • 4柴本甫，病理生理学.下，1984年
  • 5汤雪明，中华医学杂志，1982年，62卷，18页
  • 6汤雪明，中华医学杂志，1980年，60卷，385页

共引文献29

同被引文献20

  • 1Hart J, Sachdev PS, Sidhu KS. A combined epigenetic and non- genetic approach for reprogramming human somatic cells [ J ] . PLoS One, 2010, 5 ( 8 ) : e12297.
  • 2Taranger CK, Noer A, Sorensen al. et al. Induction of dedifferentia- tion, genome-wide transcriptional programming, and epigenetic reprogramming by extracts of carcinoma and embryonic stem cells [ J ] . Molecular Biology of the Cell, 2004, 16 : 5719-5735.
  • 3Gurdon JB, Byrne JA. The first half century of nuclear transplanta- tion [ J ] . Proceedings of the National Academy of Sciences of the USA, 2003, 100 ( 14 ) : 8048-8052.
  • 4Wilmut I, Schnieke AE, McWhir J, et al.Viable offspring derived from fetal and adult mammalian cells [ J ] . Nature, 1997, 385 : 810-813.
  • 5McGann C J, Odelberg S J, Keating MT.Mammalian myotube dedifferentiation induced by newt regeneration extract [ J ] . Proc Natl Acad Sci USA, 2001, 98 : 13699-13704.
  • 6Hansis C, Barreto G, Maltry N, et al. Nuclear reprogramming of human somatic ceils by Xenopus egg extract requires BRGI [ J ] . Curr Biol, 2004, 14 ( 16 ) : 1475-1480.
  • 7Kwon DN, Kang MI-I, Oh MH. Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes [J ] . Development, 2013, 140 : 2468-2471.
  • 8Miyamoto K, Tsukiyama T, Yang Y. Cell-free extracts from mammalian oocytes partially induce nuclear rcprogramming in somatic cells [ J ] . Biol Repro, 2009, 80 ( 5 ) : 935-943.
  • 9Aasen T, Raya A, Barrero MJ, et al Efficient and rapid generation of induced pluripotent stem cells from human keratinoeytes [ J ]. Nat Bioteehnol, 2008, 26 ( 1 ) : 1276-1284.
  • 10Xu YN, Guan N, Wang ZD. ES cell extract-induced expression of pluripotent factors in somatic cells [ J ] . The Anatomical Record, 2009, 292 : 1229-1234.

引证文献2

组织工程与重建外科杂志
2011年 第1期

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