MP对大鼠脊髓损伤后HSP 27表达的影响

Effect of High Dose Methylprednisolone on Heat Shock Protein 27 Expression after Spinal Cord Injury

目的研究脊髓损伤后热休克蛋白27(HSP 27)的表达及甲基强的松龙(MP)对其表达的影响。方法采用改良Allen’s打击法建立大鼠急性脊髓损伤模型。随机将90只大鼠分成正常组、对照组及甲基强的松龙组。分别在脊髓损伤6 h、24 h、7 d后行脊髓病理形态学观察,用免疫组织化学染色的方法观察脊髓细胞中HSP 27的表达。用SPSS软件对数据进行统计学处理。结果在HSP 27量的表达上,3组差别有统计学意义(P<0.05)。MP治疗可明显改善损伤脊髓的病理形态。结论 MP可使具有保护作用的HSP 27表达上调,从形态学角度提示了MP可能激发并增强受损中枢神经的自我保护及修复机制。

Objective To investigate the effect of high dose Methylprednisolone（MP） on heat shock protein27（HSP27） expression after spinal cord injury.Methods 90 rats were randomly divided into three groups： normal group,control group,Methyprednisolone treatment group.Improved Allen′s weight drop model of acute spinal cord injury was prepared to study the pathological changes of spinal cord.After 6hs,24hs and 7 days,the spinal cords were taken out and cut,then the pathological changes of spinal cord cells with HE staining and the expression of HSP27 in spinal cord cells with immunohistochemical staining were observed respectively.The data were analysed with SPSS soft ware.Results ①The expression of HSP27 which was protective to central nerves in the MP treatment groups was up-regulated.The quantity of HSP27 in spinal cord cells in control groups and MP treatment groups had a difference in statistics（P〈0.05）.②The pathological changes of spinal cord cells were significantly improved in MP treatment groups.Conclusion The present results indicate that high-dose methylprednisolone treatment in an early period induces the upregulation of HSP27 and it might provocate and enhance the self-protection and self-repair of the injured central nerves in the view of morphology.