云南昭通天麻多糖含量测定

Determination of Polysaccharides Content in Zhaotong Gastrodia elsta

用苯酚-硫酸法对14个昭通天麻样品及3个外购天麻样品进行多糖含量测定。结果显示：在490nm波长处测定吸光度，在浓度0～112．0μg／mL之间线性关系良好（r=0．9973）；精密度试验，RSD：0．07％；稳定性试验，RSD=1．67％；加样回收率100．6％，RSD=1．37％；该方法简单、准确；昭通市彝良县小草坝乡一带所产的天麻样品多糖含量为10．4％～21．6％，其多糖含量普遍高于其他地区，可作为判别昭通天麻品质优良的依据之一。

Abstract ：The polysaccharides content in 14 samples of Gastrodia elsta produced in Zhaotong and 3 samples of G. elsta bought outside Yunnan Province was determined by means of applying phenol-sulfuric acid method. The absorptiophotometric determinations were carried out at 490 nm. There was a linear relationship between the photo- metric value and the concentration within the scale of 0 - 112.0 Ixg/mL （ r = 0. 997 3 ）. The RSD value of precision test was 0.07%, the RSD value of stability test was 1.67%, and the recovery rate and its RSD value were 100.6% and 1.37% respectively. The results of the study indicated that the determination of polysaccharide contents by phenol-sulfuric acid method was simple but accurate, the polysaccharides content in the G. elsta produced in Xiaocaoba Township of Yiliang County, Zhaotong Prefecture was 10.4% - 21.6%, which was generally higher than those produced from other regions. The polysaccharides content might be adopted as one of the indexes to identify the quality of G. elsta produces.