施氮和接种 AM 真菌对刺槐生长及营养代谢的影响

Effects of Nitrogen and AM Fungi on the Growth and Nutrition Metabolism of Robinia pseudoacacia

以灭菌土为生长基质,采用盆栽试验和室内分析相结合的方法,研究不同施氮水平下接种AM真菌摩西球囊霉和缩球囊霉对刺槐生长和营养代谢的影响。结果表明:接种AM真菌后刺槐根系菌根侵染率、根系活力和植株的生物量以及叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和硝酸还原酶(NR)活性都随着施氮量的增加先升高后降低,在土壤中施氮量为0.4g·kg-1水平时最好;相反,刺槐叶片丙二醛(MDA)和游离脯氨酸(PRO)含量却随着施氮量的增加先降低后升高,在施氮量为0.4g·kg-1水平时最小。AM真菌的菌根效应因菌种不同而异,接种摩西球囊霉后,其菌根侵染率与各指标的相关性显著,更能促进刺槐的生长和营养代谢。

The growth and nutrition metabolism of Robinia pseudoacacia were investigated by inoculating with the Glomus mosseae and G.constrictum under different nitrogen levels in sterilized soil.The results showed that the colonization,root system vigor,biomass,leaf superoxidedismutase （SOD）,catalase （CAT） and nitrate reductase （NR） activity were significantly increased with inoculation with AM fungi,and the parameters were all influnced by the nitrogen levels,increasing and then decreasing with the increasing nitrogen levels,with the peak at 0.4 g·kg ^-1.To the contrast,the contents of malondialdehyde （MDA） and proline （PRO） were decreased first and then increased with the increasing nitrogen levels,with the lowest value at 0.4 g·kg ^-1.Effects of mycorrhiza varied by different AM fungi.Inoculation with G.mosseae has a significant correlation with infection rate and other indices,which promoted the growth and nutrition metabolism of Robinia pseudoacacia.